Περίληψη:
Study objective: The aim of this study was to examined whether
transforming growth factor (TGF)-beta(3) induces pleurodesis in rabbits
and collagen production by human pleural mesothelial cells in vitro.
Design: A combined animal and in vitro study.
Methods: TGF-beta(3) was injected intrapleurally in rabbits at the
following doses: 0.167 mu g, 0.5 mu g, 1.67 mu g, and 5 mu g (five
rabbits per group). The rabbits were killed 14 days following injection,
and pleurodesis was graded from 1 (none) to 8 (complete symphysis).
Pleural mesothelial cell cultures were established from benign pleural
effusions and treated with TGF-beta(3) for 48 h at the following doses:
0 (control), 5 ng/mL, 1.5 ng/mL, 5 ng/mL, and 15 ng/mL and 50 ng/mL (two
independent experiments). Collagen I messenger RNA (mRNA) expression was
quantified using real-time polymerase chain reaction.
Results: The median pleurodesis score was 5 (interquartile range
[IQR], 4.5) in the 0.167-mu g group, 6 (IQR, 5) in the 0.5-mu g group,
8 (IQR, 1) in the 1.67-mu g group, and 8 (IQR, 0.5) in the 5-mu g group
(p = 0.012). Higher TGF-beta(3) doses induced the production of
significantly more pleural fluid than the lower doses (p = 0.005). The
pleural fluid induced by higher doses contained and lactate
dehydrogenase levels significantly lower numbers of nucleated cells (p =
0.014) (p = 0.013) than the pleural fluid induced by lower doses of the
agent. TGF-beta(3) markedly enhanced collagen I mBNA expression by the
human pleural mesothelial cells. This effect peaked at 5 ng/mL
TGF-beta(3). With this dose of TGF-beta(3), the collagen I mRNA
expression was increased 16-fold over control levels.
Conclusion: TGF-beta(3) causes a dose-dependent pleurodesis when
administered intrapleurally in rabbits, and induces collagen messenger
RNA synthesis from human pleural mesothelial cells.
Συγγραφείς:
Kalomenidis, I
Guo, YB
Lane, KB
Hawthorne, M
Light, RW
