Τίτλος:
Molecular and proteomic characterization of human mesenchymal stem cells derived from amniotic fluid: Comparison to bone marrow mesenchymal stem cells
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Human mesenchymal stem cells (hMSCs) constitute a population of multipotent adherent cells able to give rise to multiple mesenchymal lineages such as osteoblasts, adipocytes, or chondrocytes. So far, the most common source of MSCs has been the bone marrow (BM); however BM-MSC harvesting and processing exhibits major drawbacks and limitations. Thus, identification and characterization of alternative sources of MSCs are of great importance. In the present study, we isolated and expanded fetal MSCs from second-trimester amniotic fluid (AF). We documented that these cells are of embryonic origin, can differentiate under appropriate conditions into cell types derived from all three germ layers, and express the pluripotency marker Oct-4, the human Nanog protein, and the stage-specific embryonic antigen-4 (SSEA-4). Furthermore, we systematically tested the immunophenotype of cultured MSCs by flow cytometry analysis using a wide variety of markers. Direct comparison of this phenotype to the one derived from cultured BM-MSCs demonstrated that cultured MSCs from both sources exhibit similar expression patterns. Using the two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) approach, we have generated for the first time the protein map of cultured AF-MSCs by identifying 261 proteins, and we compared it directly to that of cultured BM-MSCs. The functional pattern of the identified proteins from both sources was similar. However, cultured AF-MSCs displayed a number of unique proteins related to proliferation and primitive phenotype, which may confer to the distinct features of the two types. Considering the easy access to this new cell source and the yield of expanded MSCs for stem cell research, AF may provide an excellent source of MSCs both for basic research and for potential therapeutic applications. © 2007 Mary Ann Liebert, Inc.
Συγγραφείς:
Roubelakis, M.G.
Pappa, K.I.
Bitsika, V.
Zagoura, D.
Vlahou, A.
Papadaki, H.A.
Antsaklis, A.
Anagnou, N.P.
Περιοδικό:
Stem Cells and Development
Λέξεις-κλειδιά:
octamer transcription factor 4; stage specific embryo antigen 4; transcription factor NANOG, amnion fluid; article; cell differentiation; cell proliferation; controlled study; flow cytometry; hematopoietic stem cell; human; human cell; immunophenotyping; matrix assisted laser desorption ionization time of flight mass spectrometry; mesenchymal stem cell; priority journal; proteomics; two dimensional gel electrophoresis, Amniocentesis; Amniotic Fluid; Antigens, CD; Bone Marrow Cells; Cell Culture Techniques; Cell Differentiation; Cell Division; Female; Humans; Kinetics; Mesenchymal Stem Cells; Pregnancy; Proteomics; Reverse Transcriptase Polymerase Chain Reaction
DOI:
10.1089/scd.2007.0036
