Τίτλος:
Expression of oncofetal RNA-binding protein CRD-BP/IMP1 predicts clinical outcome in colon cancer
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The oncofetal CRD-BP/IMP1 RNA binding protein regulates post-transcriptionally a handful of RNA transcripts, implicated in cell adhesion and invadopodia formation and was recently identified as a target of the β-catenin/Tcf transcription factor that is constitutively activated in colorectal carcinomas (CRCs). The expression of CRD-BP/IMP1 was studied in normal adult intestines and CRCs. In normal mucosa, CRD-BP/IMP1 immunoreactivity was observed in few scattered cells located predominantly at or near the bottom of the crypts, whereas in CRCs the protein was detectable in tumor cells of 50% of the specimens analyzed. CRD-BP/ IMP1 mRNA expression was measured by qRT-PCR in 78 CRCs. Thirty-two (41%) of the specimens were negative or had negligible expression, whereas the remaining forty-six (59%) expressed a wide range of CRD-BP/IMP1 mRNA levels. CRD-BP/IMP1 mRNA expression correlated with that of the putative stem/progenitor cell marker Musashi-1 mRNA (p = 0. 035). CRD-BP/IMP1 positive tumors metastasized and/or recurred more frequently (p = 0.001) and its expression defined a group of patients with shorter survival (p = 0.014). Furthermore, in a multivariate analysis CRD-BP/IMP1 expression was found to be an independent predictor of survival (p = 0.015). For stage I & II patients, the differences in metastasis/recurrence and survival rates remained significant (p = 0.001 and 0.033, respectively). These findings indicate that CRD-BP/IMP1 positive tumors exhibit early disease dissemination and unfavorable prognosis. © 2007 Wiley-Liss, Inc.
Συγγραφείς:
Dimitriadis, E.
Trangas, T.
Milatos, S.
Foukas, P.G.
Gioulbasanis, I.
Courtis, N.
Nielsen, F.C.
Pandis, N.
Dafni, U.
Bardi, G.
Ioannidis, P.
Περιοδικό:
International Journal of Cancer
Λέξεις-κλειδιά:
messenger RNA; oncofetal antigen; protein crd bp; protein imp1; RNA binding protein; unclassified drug, adult; aged; article; cancer recurrence; cancer survival; colon cancer; correlation analysis; female; human; human tissue; immunohistochemistry; intestine crypt; major clinical study; male; metastasis; outcome assessment; prediction; priority journal; protein expression; protein localization; reverse transcription polymerase chain reaction; tissue distribution, Adult; Aged; Aged, 80 and over; Colonic Neoplasms; Female; Gene Expression; Humans; Immunohistochemistry; Intestinal Mucosa; Male; Middle Aged; Prognosis; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; RNA-Binding Proteins
