Τίτλος:
Factors mediating lipofection potency of a series of cationic phosphonolipids in human cell lines
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
A series of cationic liposomes known as cationic phosphonolipids (CPs) were evaluated as vehicles for in vitro gene transfer in K562 erythroleukemia cells and 5637 epithelial carcinoma cells. For each CP and target cell type examined, detailed analyses were performed to determine optimal transfection conditions (lipid/ DNA (+/-) charge ratio, amount of complexed episomal DNA, liposomal and lipoplex size, complexation medium and duration of complex-cell exposure time). Lipofection conditions were determined to be both cell- and lipid-type specific. Complexation medium critically affected transfection competence. The initial size of the liposome was not always predictive of lipofection potency. The lipid chemical composition had a strong impact upon lipofection efficiency; DOPE inclusion in the liposome formulations was found to affect the levels of transgene expression in a cell-dependent way. Notably, effective transgene expression was characterized by prominent plasmid nuclear incorporation. Human Aγ- and ε-globin transgene nuclear incorporation and expression in 5637 cells post GLB.391-mediated lipofection lends credence to its use as a vehicle of therapeutic transgene delivery. © 2006 Elsevier B.V. All rights reserved.
Συγγραφείς:
Koumbi, D.
Clement, J.-C.
Sideratou, Z.
Yaouanc, J.-J.
Loukopoulos, D.
Kollia, P.
Περιοδικό:
Biochimica et Biophysica Acta. General Subjects
Λέξεις-κλειδιά:
beta galactosidase; cationic phosphonolipid; cell nucleus DNA; dioleylphosphatidylethanolamine; drug vehicle; glb 391; glb 43; glb 73; lipid; lipofectamine; liposome; luciferase; plasmid DNA; unclassified drug, article; cell viability; chemical composition; complex formation; controlled study; DNA transfection; enzyme activity; gene expression; gene transfer; human; human cell; incubation time; nonviral gene delivery system; particle size; physical chemistry; priority journal; protein expression; target cell; transgene, Cations; Cell Line, Tumor; Humans; Kinetics; Nucleic Acid Hybridization; Phospholipids; Plasmids; Transfection
DOI:
10.1016/j.bbagen.2006.03.005
