Περίληψη:
Study objectives: To establish a murine model of pneumothorax-associated
pleural eosinophilia and to examine the role of interleukin (IL)-5 and
IL-13 in the pathogenesis of this reaction.
Design: An animal study.
Interventions: One hundred thirty-seven C57/BI-6 mice were used in this
study. Wild-type animals were injected intrapleurally with 0.4 mL of air
and were killed at different time points (30 min to 7 days) after the
injection. IL-5 knockout and IL-13 knockout animals were killed 24 h and
48 h after the injection. Pleural inflammation was assessed by pleural
lavage (PL).
Measurements and results: PL cells were significantly increased
following the induction of pneumothorax. The peak number of neutrophils,
observed at 12 h, was 900 times higher than the control. The peak number
of eosinophils, observed at 48 h, was 700 times higher than the control.
Lymphocytes and mononuclear cells increased threefold and fourfold,
respectively. IL-5 knockout mice had significantly less PL eosinophils
than that the wild-type or the IL-13 knockout mice at 24 h (150 +/-
46/mu L, 903 +/- 244/mu L, and 912 +/- 168/mu L, respectively; p =
0.013) and 48 h (181 +/- 45/mu L, 1,587 +/- 212/mu L, and 1,379 +/-
364/mu L, respectively; p = 0.003).
Conclusion: Pneumothorax induces an inflammatory reaction of the mouse
pleura, mainly characterized by increased neutrophils and eosinophils.
IL-5 but not IL-13 is required for pneumothorax-associated pleural
eosinophilia.
Συγγραφείς:
Kalomenidis, I
Guo, YB
Peebles, RS
Lane, KB
Papiris, S
and Elias, J
Light, RW
