Τίτλος:
Evaluation of the clinical sensitivity for the quantification of human
immunodeficiency virus type 1 RNA in plasma: Comparison of the new COBAS
TaqMan HIV-1 with three current HIV-RNA assays-LCx HIV RNA quantitative,
VERSANT HIV-1 RNA 3.0 (bDNA) and COBAS AMPLICOR HIV-1 Monitor v1.5
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The COBAS TaqMan HIV-1 test (Roche Diagnostics) was compared with the
LCx HIV RNA quantitative assay (Abbott Laboratories), the Versant HIV-1
RNA 3.0 (bDNA) assay (Bayer) and the COBAS Amplicor HIV-1 Monitor v1.5
test (Roche Diagnostics), using plasma samples of various viral load
levels from HIV-1-infected individuals. In the comparison of TaqMan with
LCx, TaqMan identified as positive 77.5% of the 240 samples versus
72.1% identified by LCx assay, while their overall agreement was 94.6%
and the quantitative results of samples that were positive by both
methods were strongly correlated (r = 0.91). Similarly, in the
comparison of TaqMan with bDNA 3.0, both methods identified 76.3% of
the 177 samples as positive, while their overall agreement was 95.5%
and the quantitative results of samples that were positive by both
methods were strongly correlated (r = 0.95). Finally, in the comparison
of TaqMan with Monitor v 1.5, TaqMan identified 79.5% of the 156
samples as positive versus 80.1% identified by Monitor v 1.5, while
their overall agreement was 95.5% and the quantitative results of
samples that were positive by both methods were strongly correlated (r =
0.96). In conclusion, the new COBAS TaqMan HIV-1 test showed excellent
agreement with other widely used commercially available tests for the
quantitation of HIV-1 viral load. (c) 2005 Elsevier B.V. All rights
reserved.
Συγγραφείς:
Katsoulidou, A
Petrodaskalaki, M
Sypsa, V
Papachristou, E
and Anastassopoulou, CG
Gargalianos, P
Karafoulidou, A and
Lazanas, M
Kordossis, T
Andoniadou, A
Hatzakis, A
Περιοδικό:
Journal of Virological Methods
Εκδότης:
ELSEVIER SCIENCE BV
Λέξεις-κλειδιά:
HIV-1 RNA; viral load; cobas TaqMan; LCx; branched DNA; COBAS amplicor
monitor v1.5
DOI:
10.1016/j.jviromet.2005.07.014
