Περίληψη:
Background: Platelet-activating factor (PAF) is an endogenous lipid
mediator that lays a key role in catalyzing various pro-inflammatory
processes associated with acute liver injury. In the present study, the
possible influence of PAF-R antagonist (BN52021) on the protection of
liver injury after 4-hydroxyacetanilide, N-acetyl-p-aminophenol,
paracetamol (APAP) intoxication was investigated. Methods: Thereby, one
group of rats was treated with a toxic dose of APAP (3.5 g/kg body
weight (b.w.). The animals were killed at 56, 66, 72, 84 and 96 h after
treatment. Results: APAP was found to cause an acute hepatic injury,
evident by alterations of biochemical (serum enzymes: aspartate
aminotransferase (AST), alanine aminotransferase (ALT) and alkaline
phosphatase) and liver histopathological (degree of necrosis and
apoptosis) indices, which was followed by liver regeneration, evident by
three independent indices ([H-3] thymidine incorporation into hepatic
DNA, liver thymidine kinase activity and hepatocyte mitotic index). The
protective effects of BN52021 were qualified during post-treatment time
by: (1) significant reduction of hepatic injury as showed by all
biochemical and histological parameters, (2) high decrease of
regenerating activity showed by three regenerative markers and (3)
remarkable increase of PAF-acetylhydrolase (PAF-AH) activity.
Conclusion: These results suggest that PAF may play an important role in
APAP-induced liver injury and regeneration, and PAF-R antagonist
(BN52021) attenuates liver damage.
Συγγραφείς:
Grypioti, AD
Theocharis, SE
Demopoulos, CA and
Papadopoulou-Daifoti, Z
Basayiannis, AC
Mykoniatis, MG
