Τίτλος:
Fibrillin expression and localization in various types of carcinomas of
the thyroid gland
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Fibrillin is an extracellular matrix (ECM) glycoprotein, a main
component of microfibrills, suggested to support cell attachment and to
impact cell differentiation and migration. The aim of this study was to
investigate fibrillin-1 expression in thyroid carcinomas at mRNA and
protein level, since ECM proteins are suggested to be of great
importance for the metastatic potential of carcinomas. RNA was extracted
from 13 thyroid carcinoma cell lines and RT-PCR analysis with
gene-specific primers revealed fibrillin-1 mRNA expression in all cell
lines, with highest expression in the follicular carcinoma cell line WRO
and lowest expression in the two anaplastic cell lines ( APO, FRO).
Furthermore, we investigated fibrillin-1 expression by
immumohistochemistry in a commercially available tissue microarray
including 50 thyroid carcinomas as well as in archival tissue from 33
thyroid carcinomas. Fibrillin-1 demonstrated a cytoplasmic location in
the neoplastic cells of almost all carcinomas apart from the follicular
ones. The most intense staining was observed in papillary carcinomas
with some evidence of a slight increased intensity in advanced stages.
Our data indicate that fibrillin-1 is strongly expressed by the
neoplastic cells of thyroid carcinomas in different degree in the
various histologic types and might be implicated in cell-stroma
interaction in terms of signaling, attachment and migration.
Συγγραφείς:
Tseleni-Balafouta, S
Gakiopoulou, H
Fanourakis, G
Voutsinas,
G
Litsiou, H
Sozopoulos, E
Balafoutas, D
Patsouris, E
Περιοδικό:
Modern Pathology
Εκδότης:
Nature Publishing Group
Λέξεις-κλειδιά:
fibrillin-1; RT-PCR; thyroid carcinomas; tissue microarray;
extracellular matrix
DOI:
10.1038/modpathol.3800578
