Τίτλος:
Periostin is identified as a putative metastatic marker in breast cancer-derived exosomes
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Breast cancer (BrCa) is the most frequent cancer type in women and a leading cause of cancer related deaths in the world. Despite the decrease in mortality due to better diagnostics and palliative care, there is a lack of prognostic markers of metastasis. Recently, the exploitation of liquid biopsies and in particular of the extracellular vesicles has shown promise in the identification of such prognostic markers. In this study we compared the proteomic content of exosomes derived from metastatic and nonmetastatic human (MCF7 and MDA-MB-231) and mouse (67NR and 4T1) cell lines. We found significant differences not only in the amount of secreted exosomes but most importantly in the protein content of exosomes secreted from metastatic versus nonmetastatic ones. We identified periostin as a protein that is enriched in exosomes secreted by metastatic cells and validated its presence in a pilot cohort of breast cancer patient samples with localized disease or lymph node (LN) metastasis.
Συγγραφείς:
Vardaki, I.
Ceder, S.
Rutishauser, D.
Baltatzis, G.
Foukakis, T.
Panaretakis, T.
Περιοδικό:
OncoTargets and therapy
Εκδότης:
Impact Journals, LLC
Λέξεις-κλειδιά:
alpha2 integrin; beta catenin; beta1 integrin; biological marker; CD81 antigen; glypican; glypican 1; glypican 4; nerve cell adhesion molecule; proton transporting adenosine triphosphate synthase; scleroprotein; transcription factor RUNX2; tumor susceptibility gene 101 protein; unclassified drug; uvomorulin; cell adhesion molecule; POSTN protein, human; proteome; tumor marker, 4T1 cell line; 67NR cell line; animal cell; Article; bioinformatics; breast cancer; breast cancer cell line; cancer grading; cancer staging; cell migration; cellular distribution; clinical article; comparative study; controlled study; exosome; female; human; human cell; lymph node metastasis; MCF 7 cell line; MDA MB 231 cell line; mouse; nonhuman; organelle size; protein analysis; protein content; protein expression; protein localization; proteomics; animal; breast tumor; cell motion; exosome; metabolism; metastasis; pathology; procedures; reproducibility; tumor cell line, Animals; Biomarkers, Tumor; Breast Neoplasms; Cell Adhesion Molecules; Cell Line, Tumor; Cell Movement; Exosomes; Female; Humans; Mice; Neoplasm Metastasis; Proteome; Proteomics; Reproducibility of Results
DOI:
10.18632/oncotarget.11663
