Τίτλος:
Th2 biased upper airway inflammation is associated with an impaired response to viral infection with herpes simplex virus
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: We aimed to elucidate possible differences in antiviral defense in chronic rhinosinusitis with nasal polyps (CRSwNP) mucosal tissue compared to healthy mucosal tissue (HMT) upon herpes simplex virus 1 (HSV1) exposure. Methodology: HMT and CRSwNP samples were infected with HSV1. We visualized the virus location by immunofluorescence and monitored invasion by a score. The mediators Interferon (IFN)-α, IFN-β, IFN-λ, IFN-γ, Interleukin (IL)-6, IL-1β, Tumor necrosis factor (TNF)-α, IL-17, IL-5, IL-10 were measured in culture supernatants at baseline and at 24h, 48h and 72h after virus incubation. Results: CRSwNP mucosal tissue showed a significant deficit in IFN-γ and IL-17 release within 24 to 72 hours after infection in comparison to HMT, at the same time releasing significantly more pro-inflammatory cytokines including IL-1β and TNF-α. These findings were associated with significantly higher viral invasion scores at 48 and 72 h in CRSwNP mucosa compared to those for the HMT. Conclusions: We demonstrate for the first time in a human ex-vivo mucosal model that the inadequate response of CRSwNP may be associated with a deeper intrusion of viruses into the mucosal tissue, and may contribute to more and longer symptoms upon acute infection, but also to the persistence of inflammation in CRSwNP tissue. © 2016, AMC. All rights reserved.
Συγγραφείς:
Lan, F.
Wang, X.D.
Nauwynck, H.J.
Holtappels, G.
Zhang, L.
Johnston, S.L.
Papadopoulos, N.G.
Bachert, C.
Zhang, N.
Λέξεις-κλειδιά:
cytokine; IL10 protein, human; IL1B protein, human; IL5 protein, human; IL6 protein, human; immunoglobulin E; interferon; interleukin 10; interleukin 17; interleukin 1beta; interleukin 5; interleukin 6; TNF protein, human; tumor necrosis factor, adolescent; adult; aged; case control study; chronic disease; enzyme linked immunosorbent assay; female; herpes simplex; Herpes simplex virus 1; human; immunology; inflammation; male; middle aged; nose mucosa; nose polyp; rhinitis; sinusitis; Th2 cell; young adult, Adolescent; Adult; Aged; Case-Control Studies; Chronic Disease; Cytokines; Enzyme-Linked Immunosorbent Assay; Female; Herpes Simplex; Herpesvirus 1, Human; Humans; Immunoglobulin E; Inflammation; Interferons; Interleukin-10; Interleukin-17; Interleukin-1beta; Interleukin-5; Interleukin-6; Male; Middle Aged; Nasal Mucosa; Nasal Polyps; Rhinitis; Sinusitis; Th2 Cells; Tumor Necrosis Factor-alpha; Young Adult
