Τίτλος:
Inherent Immune Cell Variation within Colonic Segments Presents Challenges for Clinical Trial Design
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background and Aims: Intestinal biopsy sampling during IBD trials represents a valuable adjunct strategy for understanding drug responses at the tissue level. Given the length and distinctive embryonic origins of the proximal and distal colon, we investigated whether inherent regional differences of immune cell composition could introduce confounders when sampling different disease stages, or pre/post drug administration. Here, we capitalise on novel mass cytometry technology to perform deep immunophenotyping of distinct healthy colonic segments, using the limited numbers of biopsies that can be harvested from patients. Methods: Biopsies [2.8 mm] were collected from the caecum, transverse colon, descending colon, and rectum of normal volunteers. Intestinal leukocytes were isolated, stained with a panel of 37 antibodies, and mass cytometry data acquired. Results: Site-specific patterns of leukocyte localisation were observed. The proximal colon featured increased CD8+ T cells [particularly resident memory], monocytes, and CD19+ B cells. Conversely, the distal colon and rectum tissues exhibited enrichment for CD4+ T cells and antibody-secreting cells. The transverse colon displayed increased abundance of both γδT cells and NK cells. Subsets of leukocyte lineages also displayed gradients of expression along the colon length. Conclusions: Our results show an inherent regional immune cell variation within colonic segments, indicating that regional mucosal signatures must be considered when assessing disease stages or the prospective effects of trial drugs on leukocyte subsets. Precise protocols for intestinal sampling must be implemented to allow for the proper interpretation of potential differences observed within leukocyte lineages present in the colonic lamina propria. © 2020 The Author(s) 2020. Published by Oxford University Press on behalf of European Crohn's and Colitis Organisation. All rights reserved.
Συγγραφείς:
Tyler, C.J.
Guzman, M.
Lundborg, L.R.
Yeasmin, S.
Perez-Jeldres, T.
Yarur, A.
Behm, B.
Dulai, P.S.
Patel, D.
Bamias, G.
Rivera-Nieves, J.
Περιοδικό:
Journal of Crohn's and Colitis
Εκδότης:
Oxford University Press
Λέξεις-κλειδιά:
CD19 antigen; CD19 antigen, adult; antibody secreting cell; Article; ascending colon; B lymphocyte; CD4+ T lymphocyte; CD8+ T lymphocyte; cecum; cellular distribution; clinical trial (topic); colon; descending colon; gamma delta T lymphocyte; human; human cell; human tissue; immunocompetent cell; intestine biopsy; leukocyte; mass cytometry; memory T lymphocyte; monocyte; natural killer cell; priority journal; rectum; study design; transverse colon; biopsy; CD4+ T lymphocyte; CD8+ T lymphocyte; cellular immunity; clinical trial (topic); drug effect; female; immunology; inflammatory bowel disease; intestine mucosa; male; monocyte; pathology; patient acuity; patient selection; procedures, Adult; Antigens, CD19; B-Lymphocytes; Biopsy; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Clinical Trials as Topic; Female; Humans; Immunity, Cellular; Inflammatory Bowel Diseases; Intestinal Mucosa; Male; Monocytes; Patient Acuity; Patient Selection
DOI:
10.1093/ecco-jcc/jjaa067
