Τίτλος:
Single nucleotide polymorphisms of Toll-like receptor-4 and of autophagy-related gene 16 like-1 gene for predisposition of premature delivery: A prospective study
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
To investigate the impact of carriage of single nucleotide polymorphisms (SNPs) of the Toll-like receptor-4 (TLR4) and of autophagy-related gene 16-like-1 (ATG16L1) in preterm delivery (PTD).A prospective cohort of 145 pregnant women was studied. Women were prospectively followed-up until delivery. Genotyping for rs4986790 (Asp299Gly transition) and rs4986791 (Thr399Ile transition) of TLR4 and for rs2241880 of ATG16L1 was done by PCR-restriction fragment length polymorphism. The primary study endpoint was the impact of carriage of minor alleles of TLR4 on early PTD before gestational week 32. Associations with human chorionic gonadotrophin (hCG) were also analyzed. Peripheral blood mononuclear cells were isolated from 15 healthy women and stimulated for cytokine production.No difference in clinical characteristics was observed between women delivering full term and preterm. The frequency of early PTD was 25% among women carrying minor alleles of TLR4 and 6.8% among women carrying major alleles (P:.032). Odds ratios for PTD were 3.85 among women carrying the GG genotype of rs2241880 and major alleles of TLR4 and 0.26 among carriers of GG genotype and minor alleles of TLR4 (P:.030). The co-presence of GG genotype of rs2241880 and hCG above 70 U/L was an independent variable for PTD. Stimulated production of interleukin-6 was greater among women with GG genotypes of rs2241880.Minor alleles of SNPs of TLR4 predispose to early PTD. The GG genotype of rs2241880 of ATG16L1 is associated with PTD when hCG is supra-elevated. Copyright © 2019 the Author(s). Published by Wolters Kluwer Health, Inc.
Συγγραφείς:
Liassides, C.
Papadopoulos, A.
Siristatidis, C.
Damoraki, G.
Liassidou, A.
Chrelias, C.
Kassanos, D.
Giamarellos-Bourboulis, E.J.
Περιοδικό:
Medicine (United States)
Εκδότης:
Lippincott Williams and Wilkins
Λέξεις-κλειδιά:
chorionic gonadotropin; interleukin 6; toll like receptor 4; ATG16L1 protein, human; autophagy related protein; chorionic gonadotropin; cytokine; TLR4 protein, human; toll like receptor 4, adult; allele; Article; autophagy related gene 16 like 1 gene; cohort analysis; cytokine production; disease predisposition; female; follow up; gene; human; major clinical study; peripheral blood mononuclear cell; polymerase chain reaction restriction fragment length polymorphism; pregnant woman; premature labor; priority journal; prospective study; single nucleotide polymorphism; amplified fragment length polymorphism; autophagy; biosynthesis; blood; gene frequency; genetic predisposition; genetics; genotype; gestational age; physiology; pregnancy; prematurity; single nucleotide polymorphism, Adult; Amplified Fragment Length Polymorphism Analysis; Autophagy; Autophagy-Related Proteins; Chorionic Gonadotropin; Cytokines; Female; Gene Frequency; Genetic Predisposition to Disease; Genotype; Gestational Age; Humans; Polymorphism, Single Nucleotide; Pregnancy; Premature Birth; Prospective Studies; Toll-Like Receptor 4
DOI:
10.1097/MD.0000000000017313
