Τίτλος:
Immune cell response to strenuous resistive breathing: Comparison with whole body exercise and the effects of antioxidants
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background/hypothesis: Whole body exercise (WBE) changes lymphocyte subset percentages in peripheral blood. Resistive breathing, a hallmark of diseases of airway obstruction, is a form of exercise for the inspiratory muscles. Strenuous muscle contractions induce oxidative stress that may mediate immune alterations following exercise. We hypothesized that inspiratory resistive breathing (IRB) alters peripheral blood lymphocyte subsets and that oxidative stress mediates lymphocyte subpopulation alterations following both WBE and IRB. Patients and methods: Six healthy nonathletes performed two WBE and two IRB sessions for 45 minutes at 70% of VO2 maximum and 70% of maximum inspiratory pressure (Pimax), respectively, before and after the administration of antioxidants (vitamins E, A, and C for 75 days, allopurinol for 30 days, and N-acetylcysteine for 3 days). Blood was drawn at baseline, at the end of each session, and 2 hours into recovery. Lymphocyte subsets were determined by flow cytometry. Results: Before antioxidant supplementation at both WBE end and IRB end, the natural killer cell percentage increased, the T helper cell (CD3+ CD4+) percentage was reduced, and the CD4/CD8 ratio was depressed, a response which was abolished by antioxidants only after IRB. Furthermore, at IRB end, antioxidants promoted CD8+ CD38+ and blunted cytotoxic T-cell percentage increase. CD8+ CD45RA+ cell percentage changes were blunted after antioxidant supplementation in both WBE and IRB. Conclusion: We conclude that IRB produces (as WBE) changes in peripheral blood lymphocyte subsets and that oxidative stress is a major stimulus predominantly for IRB-induced lymphocyte subset alterations. © 2018 Asimakos et al.
Συγγραφείς:
Asimakos, A.
Toumpanakis, D.
Karatza, M.-H.
Vasileiou, S.
Katsaounou, P.
Mastora, Z.
Vassilakopoulos, T.
Περιοδικό:
International Journal of COPD
Εκδότης:
Dove Medical Press Ltd
Λέξεις-κλειδιά:
acetylcysteine; allopurinol; alpha tocopherol; antioxidant; ascorbic acid; malonaldehyde; retinol; antioxidant; biological marker; CD56 antigen; Fc receptor; HLA DR antigen; NCAM1 protein, human; receptor type tyrosine protein phosphatase C, adult; Article; breathing pattern; CD3+ T lymphocyte; CD4 CD8 ratio; CD4+ T lymphocyte; CD8+ T lymphocyte; cellular immunity; cytotoxic T lymphocyte; drug effect; exercise; flow cytometry; high performance liquid chromatography; human; human cell; human experiment; in vivo study; inspiratory resistive breathing; lymphocyte subpopulation; male; maximal inspiratory pressure; morning dosage; natural killer cell; normal human; oxidative stress; peripheral lymphocyte; single drug dose; venous oxygen tension; vitamin supplementation; whole body exercise; airway resistance; blood; breathing; breathing exercise; clinical trial; comparative study; drug effect; immunology; immunophenotyping; lung; lymphocyte count; metabolism; phenotype; procedures, Adult; Airway Resistance; Antioxidants; Biomarkers; Breathing Exercises; CD4-Positive T-Lymphocytes; CD56 Antigen; CD8-Positive T-Lymphocytes; Exercise; Flow Cytometry; HLA-DR Antigens; Humans; Immunophenotyping; Leukocyte Common Antigens; Lung; Lymphocyte Count; Male; Malondialdehyde; Phenotype; Receptors, IgG; Respiration
DOI:
10.2147/COPD.S154533
