Τίτλος:
Tregs restrain dendritic cell autophagy to ameliorate autoimmunity
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Design of efficacious Treg-based therapies and establishment of clinical tolerance in autoimmune diseases have proven to be challenging. The clinical implementation of Treg immunotherapy has been hampered by various impediments related to the stability and isolation procedures of Tregs as well as the specific in vivo targets of Treg modalities. Herein, we have demonstrated that Foxp3+ Tregs potently suppress autoimmune responses in vivo through inhibition of the autophagic machinery in DCs in a cytotoxic T-lymphocyte-associated protein 4-dependent (CTLA4-dependent) manner. Autophagydeficient DCs exhibited reduced immunogenic potential and failed to prime autoantigen-specific CD4+ T cells to mediate autoimmunity. Mechanistically, CTLA4 binding promoted activation of the PI3K/Akt/mTOR axis and FoxO1 nuclear exclusion in DCs, leading to decreased transcription of the autophagy component microtubule-associated protein 1 light chain 3β (Lc3b). Human DCs treated with CTLA4-Ig, a fusion protein composed of the Fc region of IgG1 and the extracellular domain of CTLA4 (also known as abatacept, marketed as Orencia), demonstrated reduced levels of autophagosome formation, while DCs from CTLA4-Ig-treated rheumatoid arthritis patients displayed diminished LC3B transcripts. Collectively, our data identify the canonical autophagy pathway in DCs as a molecular target of Foxp3+ Treg-mediated suppression that leads to amelioration of autoimmune responses. These findings may pave the way for the development of therapeutic protocols that exploit Tregs for the treatment of autoimmunity as well as diseases in which disturbed tolerance is a common denominator.
Συγγραφείς:
Alissafi, T.
Banos, A.
Boon, L.
Sparwasser, T.
Ghigo, A.
Wing, K.
Vassilopoulos, D.
Boumpas, D.
Chavakis, T.
Cadwell, K.
Verginis, P.
Περιοδικό:
JOURNAL OF CLINICAL INVESTIGATION
Εκδότης:
American Society for Clinical Investigation
Λέξεις-κλειδιά:
autoantigen; cytotoxic T lymphocyte antigen 4; hybrid protein; mammalian target of rapamycin; microtubule associated protein 1; phosphatidylinositol 3 kinase; protein kinase B; transcription factor FKHR; transcription factor FOXP3; CTLA4 protein, human; Ctla4 protein, mouse; cytotoxic T lymphocyte antigen 4; MAP1LC3 protein, mouse; MAP1LC3B protein, human; microtubule associated protein, animal experiment; animal model; antigen presentation; Article; autoimmunity; autolysosome; autophagy; dendritic cell; experimental autoimmune encephalomyelitis; female; human; human cell; immune response; immunoregulation; in vivo study; mouse; nonhuman; priority journal; protein binding; regulatory T lymphocyte; rheumatoid arthritis; animal; autophagy; dendritic cell; genetics; immunology; knockout mouse; regulatory T lymphocyte, Animals; Autoimmunity; Autophagy; CTLA-4 Antigen; Dendritic Cells; Humans; Mice; Mice, Knockout; Microtubule-Associated Proteins; T-Lymphocytes, Regulatory
