Τίτλος:
Gallium-68 labeled iron oxide nanoparticles coated with 2,3-dicarboxypropane-1,1-diphosphonic acid as a potential PET/MR imaging agent: A proof-of-concept study
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The aim of this study was to develop a dual-modality PET/MR imaging probe by radiolabeling iron oxide magnetic nanoparticles (IONPs), surface functionalized with water soluble stabilizer 2,3-dicarboxypropane-1,1-diphosphonic acid (DPD), with the positron emitter Gallium-68. Magnetite nanoparticles (Fe3O4 MNPs) were synthesized via coprecipitation method and were stabilized with DPD. The Fe3O4-DPD MNPs were characterized based on their structure, morphology, size, surface charge, and magnetic properties. In vitro cytotoxicity studies showed reduced toxicity in normal cells, compared to cancer cells. Fe3O4-DPD MNPs were successfully labeled with Gallium-68 at high radiochemical purity (>91%) and their stability in human serum and in PBS was demonstrated, along with their further characterization on size and magnetic properties. The ex vivo biodistribution studies in normal Swiss mice showed high uptake in the liver followed by spleen. The acquired PET images were in accordance with the ex vivo biodistribution results. Our findings indicate that Ga68-Fe3O4-DPD MNPs could serve as an important diagnostic tool for biomedical imaging. © 2017 Maria-Argyro Karageorgou et al.
Συγγραφείς:
Karageorgou, M.-A.
Vranješ-Djurić, S.
Radović, M.
Lyberopoulou, A.
Antić, B.
Rouchota, M.
Gazouli, M.
Loudos, G.
Xanthopoulos, S.
Sideratou, Z.
Stamopoulos, D.
Bouziotis, P.
Tsoukalas, C.
Περιοδικό:
Contrast Media and Molecular Imaging
Λέξεις-κλειδιά:
butedronic acid; gallium 68; magnetite nanoparticle; phosphate buffered saline; ultrasmall superparamagnetic iron oxide; bisphosphonic acid derivative; contrast medium; ferric ion; ferric oxide; gallium; nanoparticle, animal experiment; animal tissue; Article; controlled study; cytotoxicity; drug blood level; drug distribution; drug stability; drug synthesis; drug tissue level; drug uptake; embryo; ex vivo study; human; human cell; in vitro study; limit of quantitation; liver; mouse; nonhuman; nuclear magnetic resonance imaging; positron emission tomography; precipitation; priority journal; spleen; animal; chemistry; HEK293 cell line; isotope labeling; nuclear magnetic resonance imaging; positron emission tomography; procedures; proof of concept, Animals; Contrast Media; Diphosphonates; Ferric Compounds; Gallium Radioisotopes; HEK293 Cells; Humans; Isotope Labeling; Magnetic Resonance Imaging; Mice; Nanoparticles; Positron-Emission Tomography; Proof of Concept Study
DOI:
10.1155/2017/6951240
