Τίτλος:
Evaluation analysis of miRNAs overexpression in Liquid-Based Cytology endometrial samples
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: miRNAs have an important role as their deregulation is linked to endometrial cancer. Methods: A custom miScript® miRNA PCR Array was used to investigate for the first time the expression of eight miRNAs in forty-nine histologically confirmed Liquid Based cytology endometrial samples. The expression profile of the same miRNAs was also examined in sixty formalin-fixed tissue samples. Results: Expression of seven miRNAs was significantly higher in malignant samples with three of them (mir-182, mir-141 and mir-205) performing optimally. Conclusion: These results suggest the potential use of this non-invasive method of sampling for miRNA expression studies. Furthermore miRNA overexpression could serve as an ancillary or reflex test for optimal identification of malignant samples especially in morphologically inadequate samples. © Ivyspring International Publisher.
Συγγραφείς:
Kottaridi, C.
Spathis, A.
Margari, N.
Koureas, N.
Terzakis, E.
Chrelias, C.
Pappas, A.
Bilirakis, E.
Pouliakis, A.
Panayiotides, I.J.
Karakitsos, P.
Περιοδικό:
EUROPEAN JOURNAL OF CANCER
Εκδότης:
Ivyspring International Publisher
Λέξεις-κλειδιά:
microRNA; microRNA 141; microRNA 141 3p; microRNA 182; microRNA 182 5p; microRNA 200b 3p; microRNA 200c 3p; microRNA 205; microRNA 205 5p; microRNA 222 3p; microRNA 9 5p; unclassified drug, adult; aged; Article; comparative study; controlled study; cytology; female; gene expression profiling; histopathology; human; human tissue; male; protein expression; RNA analysis; RNA isolation; sensitivity and specificity
