Τίτλος:
Assessing the clinical value of microRNAs in formalin-fixed paraffin-embedded liposarcoma tissues: Overexpressed miR-155 is an indicator of poor prognosis
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Liposarcoma (LPS) is a malignancy with extreme heterogeneity and thus optimization towards personalizing patient prognosis and treatment is essential. Here, we evaluated miR-155, miR-21, miR-143, miR-145 and miR-451 that are implicated in LPS, as novel FFPE tissue biomarkers. A total of 83 FFPE tissue specimens from primary LPS and lipomas (LPM) were analyzed. A proteinase K incubation-Trizol treatment coupled protocol was used for RNA isolation. After polyadenylation of total RNA and reverse transcription, expression analysis of 9 candidate reference and 5 target miRNAs was performed by qPCR. Genorm and NormFinder were used for finding the most suitable molecules for normalization. Survival analyses were performed in order to evaluate the prognostic potential of miRNAs. MiR-103 and miR-191 are most suitable for normalization of miRNA expression in LPS. MiR-155 and miR-21 are clearly overexpressed (P < 0.001) in LPS compared with LPM specimens, whereas miR-145 (P < 0.001), miR-143 (P =0.008) and miR- 451 (P=0.037) are underexpressed. MiR-155 (P=0.007) and miR-21 (P=0.029) are differentially expressed between well-differentiated, dedifferentiated, myxoid/round cell and pleomorphic LPs tumor subtypes. MiR-155 represents a novel independent indicator of unfavorable prognosis in LPS (HR = 2.97, 95% CI = 1.23-7.17, P = 0.016).
Συγγραφείς:
Kapodistrias, N.
Mavridis, K.
Batistatou, A.
Gogou, P.
Karavasilis, V.
Sainis, I.
Briasoulis, E.
Scorilas, A.
Περιοδικό:
OncoTargets and therapy
Εκδότης:
Impact Journals, LLC
Λέξεις-κλειδιά:
formaldehyde; microRNA; microRNA 103; microRNA 143; microRNA 155; microRNA 191; microRNA 21; microRNA 451; paraffin; proteinase K; tumor marker; unclassified drug, adult; aged; area under the curve; Article; cancer prognosis; cancer survival; cancer tissue; cell dedifferentiation; controlled study; female; gene expression regulation; gene overexpression; human; human tissue; incubation time; liposarcoma; major clinical study; male; outcome assessment; overall survival; polyadenylation; polymerase chain reaction; quantitative analysis; reverse transcription; RNA isolation; sensitivity and specificity; Youden index
DOI:
10.18632/oncotarget.14320
