Τίτλος:
Correlation between depression, anxiety, and polymorphonuclear cells' resilience in ulcerative colitis: The mediating role of heat shock protein 70
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: To investigate whether anxiety and depression levels are associated with Heat Shock Protein 70 (HSP70) induction in the colon of patients with ulcerative colitis (UC).Methods: The design was cross-sectional. Clinical activity was assessed by the Rachmilewitz Index (CAI). Three psychometric questionnaires were used: Zung Depression Rating Scale (ZDRS), Spielberg State-Trait Anxiety Inventory (STAI), Hospital Anxiety and Depression Scale (HADS). Colon biopsies were obtained from each affected anatomical site. Severity of inflammation was assessed by eosin/hematoxylin. Constitutive (HSP70c) and inducible (HSP70i) HSP70 expression were immunohistochemically studied.Results: 29 UC patients were enrolled (69% men). Mean age was 46.5 years (SD: 19.5). Inflammation severity was moderate in 17 patients, severe in 6, and mild in 6. The mean number of years since diagnosis was 7.9 (SD: 6.5). The mean CAI was 6.4 (SD: 3.1). In active UC, there was downregulation of HSP70c in inflamed epithelium, without significant HSP70 induction. In 22/29 cases of active cryptitis, polymorphonuclear cells (PMN) clearly expressed HSP70i, with weak, focal positivity in the other 7 cases. Except for the hospital anxiety scale, scores in all psychometric tools were higher in patients with strong HSP70i immunoreactivity in the PMN. Logistic regression showed a strong positive relationship between HSP70i immunoreactivity in the PMN cells and scores in the trait anxiety, ZDRS, and hospital depression scales, (Odds ratios 1.3, 1.3, and 1.5; P = 0.018, 0.023, and 0.038; Wald test, 5.6, 5.2, and 4.3 respectively) and a weaker but significant positive correlation with the CAI (Odds ratio 1.654; P = 0.049; Wald test 3.858).Conclusion: HSP70 is induced in PMN cells of UC patients and its induction correlates with depression and anxiety levels. © 2014 Vlachos et al.; licensee BioMed Central Ltd.
Συγγραφείς:
Vlachos, I.I.
Barbatis, C.
Tsopanomichalou, M.
Abou-Assabeh, L.
Goumas, K.
Ginieri-Coccossis, M.
Economou, M.
Papadimitriou, G.N.
Patsouris, E.
Nicolopoulou-Stamati, P.
Περιοδικό:
BMC Gastroenterology
Εκδότης:
BioMed Central Ltd.
Λέξεις-κλειδιά:
heat shock protein 70; heat shock protein 70, adult; anxiety; article; clinical article; colon biopsy; controlled study; cross-sectional study; depression; disease severity; down regulation; epithelium; female; Hospital Anxiety and Depression Scale; human; human cell; human tissue; immunohistochemistry; immunoreactivity; male; polymorphonuclear cell; protein expression; protein induction; psychological rating scale; questionnaire; Rachmilewitz Index; scoring system; Spielberg State Trait Anxiety Inventory; ulcerative colitis; Zung Depression Rating Scale; aged; anxiety; colon; depression; metabolism; middle aged; neutrophil; psychology; severity of illness index; statistical model; ulcerative colitis, Adult; Aged; Anxiety; Colitis, Ulcerative; Colon; Cross-Sectional Studies; Depression; Female; HSP70 Heat-Shock Proteins; Humans; Logistic Models; Male; Middle Aged; Neutrophils; Questionnaires; Severity of Illness Index
DOI:
10.1186/1471-230X-14-77
