Τίτλος:
Human islet viability and function is maintained during high-density shipment in silicone rubber membrane vessels
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background. The shipment of human islets (IE) from processing centers to distant laboratories is beneficial for both research and clinical applications. The maintenance of islet viability and function in transit is critically important. Gas-permeable silicone rubber membrane (SRM) vessels reduce the risk of hypoxia-induced death or dysfunction during high-density islet culture or shipment. SRM vessels may offer additional advantages: they are cost-effective (fewer flasks, less labor needed), safer (lower contamination risk), and simpler (culture vessel can also be used for shipment). Method. IE were isolated from two manufacturing centers and shipped in 10-cm2 surface area SRM vessels in temperature- and pressure-controlled containers to a distant center after at least 2 days of culture (n = 6). Three conditions were examined: low density (LD), high density (HD), and a microcentrifuge tube negative control (NC). LD was designed to mimic the standard culture density for IE preparations (200 IE/cm2), while HD was designed to have a 20-fold higher tissue density, which would enable the culture of an entire human isolation in 1-3 vessels. Upon receipt, islets were assessed for viability (measured by oxygen consumption rate normalized to DNA content [OCR/DNA)]), quantity (measured by DNA), and, when possible, potency and function (measured by dynamic glucose-stimulated insulin secretion measurements and transplants in immunodeficient B6 Rag+/- mice). Postshipment OCR/DNA was not reduced in HD vs LD and was substantially reduced in the NC condition. HD islets exhibited normal function postshipment. Based on the data, we conclude that entire islet isolations (up to 400,000 IE) may be shipped using a single, larger SRM vessel with no negative effect on viability and ex vivo and in vivo function. © 2014 by Elsevier Inc. All rights reserved.
Συγγραφείς:
Kitzmann, J.P.
Pepper, A.R.
Gala-Lopez, B.
Pawlick, R.
Kin, T.
O'Gorman, D.
Mueller, K.R.
Gruessner, A.C.
Avgoustiniatos, E.S.
Karatzas, T.
Szot, G.L.
Posselt, A.M.
Stock, P.G.
Wilson, J.R.
Shapiro, A.M.
Papas, K.K.
Περιοδικό:
Transplantation Proceedings
Εκδότης:
ELSEVIER SCIENCE INC 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
Λέξεις-κλειδιά:
RAG1 protein; silastic; insulin, animal experiment; artificial membrane; cell density; cell isolation; cell viability; conference paper; controlled study; cost effectiveness analysis; DNA content; ex vivo study; human; human tissue; insulin release; mouse; nonhuman; oxygen consumption; pancreas islet cell; pancreas islet cell function; priority journal; shipping; silicone rubber membrane; animal; cell count; cell hypoxia; cell survival; culture technique; devices; laboratory diagnosis; packaging; pancreas islet; pancreas islet transplantation; physiology; secretion (process); Article; cell function; density; DNA determination; human cell, Animals; Cell Count; Cell Culture Techniques; Cell Hypoxia; Cell Survival; Humans; Insulin; Islets of Langerhans; Islets of Langerhans Transplantation; Mice; Oxygen Consumption; Product Packaging; Silicone Elastomers; Specimen Handling
DOI:
10.1016/j.transproceed.2014.06.002
