Τίτλος:
Impact of multidrug resistance on experimental empyema by Pseudomonas aeruginosa
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background:Pseudomonas aeruginosa is a cause of infections of the lower respiratory tract among patients with chronic lung disorders. It is questionable whether virulence of this species may be influenced by multidrug resistance (MDR). Objectives: To define the impact of MDR in experimental lung infection. Methods: Experimental empyema was induced in rabbits by MDR (group A, n = 16) and by susceptible isolates (group B, n = 10). Pleural fluid was sampled for quantitative culture and estimation of cell apoptosis and of tumor necrosis factor-alpha (TNFα) and malondialdehyde (MDA). Survival was recorded. Cytokine production was stimulated in U937 monocytes by samples of pleural fluid. Whole blood of rabbits was incubated with the isolates; induction of apoptosis was assessed. Results: Survival of group A was prolonged compared to group B. This was accompanied by lower bacterial counts of the inoculated pathogens in pleural fluid and in the lungs of group A compared with group B. Early apoptosis of neutrophils of pleural fluid of group A was lower compared with group B. Pleural fluid concentrations of TNFα and MDA did not differ between the groups. Cytokine production by U937 monocytes after stimulation with pleural fluid was greater in group B than in group A. The susceptible isolate induced apoptosis of neutrophils in vitro at a greater rate than the MDR isolate. Conclusions: Experimental empyema by susceptible P. aeruginosa is accompanied by greater mortality compared with MDR P. aeruginosa. This phenomenon may be attributed to the different growth pattern of the pathogens or to their interaction with the innate immune system. Copyright © 2011 S. Karger AG.
Συγγραφείς:
Giamarellos-Bourboulis, E.J.
Tzepi, I.
Tsovolou, I.
Spyridaki, A.
Tsaganos, T.
Vaki, I.
Kotsaki, A.
Polychronopoulos, V.
Περιοδικό:
RESPIRATION PHYSIOLOGY
Λέξεις-κλειδιά:
amikacin; ceftazidime; ciprofloxacin; clavulanate potassium plus ticarcillin; imipenem; interleukin 10; interleukin 1beta; malonaldehyde; meropenem; piperacillin; tumor necrosis factor alpha, animal cell; animal experiment; animal model; animal tissue; antibiotic resistance; apoptosis; article; bacterial count; bacterial growth; bacterial virulence; bacterium culture; bacterium isolate; cell stimulation; controlled study; cytokine production; empyema; experimental rabbit; growth rate; monocyte; multidrug resistance; neutrophil; nonhuman; pleura fluid; priority journal; protein content; Pseudomonas aeruginosa; Pseudomonas infection; survival rate; survival time, Animals; Bacterial Load; Cytokines; Disease Susceptibility; Drug Resistance, Multiple, Bacterial; Empyema; Humans; Immunity, Innate; Lung; Male; Malondialdehyde; Monocytes; Neutrophils; Pleural Effusion; Pseudomonas aeruginosa; Pseudomonas Infections; Rabbits; Species Specificity; Survival Rate; Tumor Necrosis Factor-alpha; U937 Cells; Virulence
