Τίτλος:
Effect of lenalidomide therapy on hematopoiesis of patients with myelodysplastic syndrome associated with chromosome 5q deletion
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: Lenalidomide improves erythropoiesis in patients with low/intermediate-1 risk myelodysplastic syndrome and interstitial deletion of the long arm of chromosome 5 [del(5q)]. The aim of this study was to explore the effect of lenalidomide treatment on the reserves and functional characteristics of bone marrow hematopoietic progenitor/precursor cells, bone marrow stromal cells and peripheral blood lymphocytes in patients with low/intermediate-1 risk myelodysplastic syndrome with del(5q). Design and Methods: We evaluated the number and clonogenic potential of bone marrow erythroid/myeloid/ megakaryocytic progenitor cells using clonogenic assays, the apoptotic characteristics and adhesion molecule expression of CD34+ cells by flow cytometry, the hematopoiesis-supporting capacity of bone marrow stromal cells using long-term bone marrow cultures and the number and activation status of peripheral blood lymphocytes in ten patients with low/intermediate-1 risk myelodysplastic syndrome with del(5q) receiving lenalidomide. Results: Compared to baseline, lenalidomide treatment significantly decreased the proportion of bone marrow CD34+ cells, increased the proportion of CD36+/GlycoA+ and CD36-/GlycoA+ erythroid cells and the percentage of apoptotic cells within these cell compartments. Treatment significantly improved the clonogenic potential of bone marrow erythroid, myeloid, megakaryocytic colony-forming cells and increased the proportion of CD34+ cells expressing the adhesion molecules CD11a, CD49d, CD54, CXCR4 and the SLAM antigen CD48. The hematopoiesis-supporting capacity of bone marrow stroma improved significantly following treatment, as demonstrated by the number of colony-forming cells and the level of stromal-derived factor-1α and intercellular adhesion molecule-1 in long-term bone marrow culture supernatants. Lenalidomide treatment also increased the proportion of activated peripheral blood T lymphocytes. Conclusions: The beneficial effect of lenalidomide in patients with lower risk myelodysplastic syndrome with del(5q) is associated with significant increases in the proportion of bone marrow erythroid precursor cells and in the frequency of clonogenic progenitor cells, a substantial improvement in the hematopoiesis-supporting potential of bone marrow stroma and significant alterations in the adhesion profile of bone marrow CD34+ cells. ©2010 Ferrata Storti Foundation.
Συγγραφείς:
Ximeri, M.
Galanopoulos, A.
Klaus, M.
Parcharidou, A.
Giannikou, K.
Psyllaki, M.
Symeonidis, A.
Pappa, V.
Kartasis, Z.
Liapi, D.
Hatzimichael, E.
Kokoris, S.
Korkolopoulou, P.
Sambani, C.
Pontikoglou, C.
Papadaki, H.A.
Περιοδικό:
Haematologica-the hematology journal
Λέξεις-κλειδιά:
alpha4 integrin; caps revlimid; CD34 antigen; CD36 antigen; CD48 antigen; chemokine receptor CXCR4 antagonist; glycolic acid; intercellular adhesion molecule 1 antibody; lenalidomide; lymphocyte function associated antigen 1; unclassified drug, adult; aged; article; bone marrow cell; chromosome 5q; chromosome deletion; clinical article; drug effect; erythroid cell; female; hematopoiesis; hematopoietic stem cell; human; human cell; male; megakaryocyte; myelodysplastic syndrome; peripheral lymphocyte, Aged; Aged, 80 and over; Antineoplastic Agents; Bone Marrow Cells; Cells, Cultured; Chromosome Deletion; Chromosomes, Human, Pair 5; Colony-Forming Units Assay; Cytokines; Female; Flow Cytometry; Hematopoiesis; Hematopoietic Stem Cells; Humans; Male; Middle Aged; Myelodysplastic Syndromes; Thalidomide
DOI:
10.3324/haematol.2009.010876
