Περίληψη:
In cystic fibrosis (CF), transcript analysis and quantification are important for diagnosis, prognosis and also as surrogate markers for some therapies including gene therapy. Classical RNA-based methods require significant expression levels in target samples for appropriate analysis, thus PCR-based methods are evolving towards reliable quantification. Various protocols for the quantitative analysis of CFTR transcripts (including those resulting from splicing variants) are described and discussed here. © 2004 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.
Συγγραφείς:
Amaral, M.D.
Clarke, L.A.
Ramalho, A.S.
Beck, S.
Broackes-Carter, F.
Rowntree, R.
Mouchel, N.
Williams, S.H.
Harris, A.
Tzetis, M.
Steiner, B.
Sanz, J.
Gallati, S.
Nissim-Rafinifa, M.
Kerem, B.
Hefferon, T.
Cutting, G.R.
Goina, E.
Pagani, F.
Λέξεις-κλειδιά:
beta actin; messenger RNA; transmembrane conductance regulator, allele; article; cystic fibrosis; diagnostic accuracy; exon; gene expression; gene identification; gene mutation; genetic variability; human; nonhuman; prognosis; quality control; quantitative analysis; real time polymerase chain reaction; reproducibility; reverse transcription polymerase chain reaction; RNA splicing; RNA transcription; species difference, Cystic Fibrosis; Cystic Fibrosis Transmembrane Conductance Regulator; Genetic Techniques; Humans; Reverse Transcriptase Polymerase Chain Reaction; Transcription, Genetic
