Τίτλος:
Characterization of the human urine proteome by preparative
electrophoresis in combination with 2-DE
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The protein components of urine are useful indicators of renal function
and human health in general. Urine samples are easily attainable making
them ideal substrates for biomarker research. Analysis of the urine
proteome however, has been hindered by the great variability of the
urine specimens, and the presence of various proteins in low abundance
or modified forms. To alleviate some of these problems urine samples
from five different individuals were pooled, concentrated and the
proteome. characterized by a combination of preparative electrophoresis
and 2-DE, followed by PMF. A total of 778 protein spots corresponding to
141 different gene products were identified. In comparison, 171 spots
corresponding to 44 unique proteins were identified in the
unfractionated starting material. Among the proteins identified from the
preparative electrophoresis were many of low abundance such as proteins
involved in signal transduction. Furthermore, the median molecular mass
of the identified proteins from the preparative electrophoresis was
significantly lower in comparison to the proteins identified from the
unfractionated starting material (39886 Da versus 71317 Da,
respectively). Concluding, application of this methodology provides a
coherent analysis of the urine proteome and contributes to the
generation of the urine protein map in health and disease.
Συγγραφείς:
Zerefos, Panagiotis G.
Vougas, Konstantinos
Dimitraki, Ploumisti
and Kossida, Sophia
Petrolekas, Andreas
Stravodimos,
Konstantinos
Giannopoulos, Aris
Fountoulakis, Michael and
Vlahou, Antonia
Περιοδικό:
Cancer Genomics and Proteomics
Λέξεις-κλειδιά:
low molecular mass proteins; preparative electrophoresis; proteome;
urine
DOI:
10.1002/pmic.200500671
