Τίτλος:
Myoepithelial cell cocktail (p63+SMA) for the evaluation of sclerosing
breast lesions
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Sclerosing breast lesions may sometimes mimic the appearance of
infiltrating carcinoma due to the entrapment of ductular structures in a
fibrotic core. The immunohistochemical detection of the outer
myoepithelial cell layer that is indicative of a non-infiltrating lesion
is a valuable clue for the diagnosis of such ambiguous cases. The
myoepithelial cell markers smooth muscle actin (SMA) and p63 are most
commonly used since their specificity and sensitivity are well
established. However, recent studies have indicated that some
morphologically distinct myoepithetial cells fait to stain for SMA and
that p63 positivity can be rarely expressed by a subset of malignant
epithelial cells. Moreover, SMA can also be positive in stromal
myofibroblastic cells and normal vessels that can be found close to the
entrapped ductules and might be erroneously interpreted as myoepithetial
cells. In this study, we used a double-immunotabeling technique
combining both SMA and p63 antibodies (myoepithelial cell cocktail), in
order to investigate whether this technique is advantageous over either
marker used alone, in diagnosing sclerosing breast lesions. Our results
indicate that p63 alone is not a useful myoepithetial cell marker if
applied in large sclerosing breast lesions, however, in smaller lesions
it is still of high value. On the contrary, SMA proved significantly
useful in the evaluation of myoepithelial cells in larger but not in
smaller complex sclerosing Lesions. The myoepithetial cell cocktail has
a staining sensitivity identical to that of SMA. Nevertheless, in a
certain number of cases the cocktail might be useful in differentiating
myoepithelial cells from stromal myofibroblasts or vascular smooth
muscle cells due to the false impression of a higher staining intensity
of the cocktail resulting from the expression of both nuclear and
cytoplasmic/membranous antibodies that occupy a wider area of the cell
under control. (c) 2005 Elsevier Ltd. All rights reserved.
Συγγραφείς:
Pavlakis, K.
Zoubouli, Chr.
Liakakos, Th.
Messini, I. and
Keramopoullos, A.
Athanassiadou, S.
Kafousi, M. and
Stathopoulos, E. N.
Εκδότης:
Churchill Livingstone
Λέξεις-κλειδιά:
SMA; p63; sclerosing breast lesions
DOI:
10.1016/j.breast.2005.11.005
