Περίληψη:
Background. Telomerase preserves telomeres’ function and structure
preventing cellular senescence. Its activity is reduced in peripheral
blood mononuclear cells (PBMC) of haemodialysis (HD) patients. The
purpose of this study is to investigate the potential correlation
between increased oxidative stress/inflammation and telomerase activity
in PBMC of HD patients.
Methods: Telomerase activity was measured by PCR-ELISA in PBMC isolated
from a group of 42 HD patients and 39 subjects with estimated glomerular
filtration rate >= 80 mL/min (control group). Serum oxidized low-density
lipoprotein (ox-LDL), tumour necrosis factor-alpha (TNF-alpha) and
interleukin-10 (IL-10) were also measured in both groups by ELISA.
Results: Ox-LDL was negatively correlated to percentage telomerase
activity in PBMC (r = -0.506, P = 0.000 in the whole group of 81 HD and
normal subjects and r = -0.559, P < 0.001 in HD patients). TNF was also
inversely associated with percentage telomerase activity in the whole
group studied (r= -0.492, P= 0.000) while IL-10 was not. In stepwise
multiple linear regression, taking into consideration the most important
characteristics of the HD patients and control group, the only
significant predictors for percentage telomerase activity in PBMC were
ox-LDL and TNF (beta = -0.421, t= -4.083, P= 0.000 and beta= -0.381, t=
-3.691, P= 0.000, respectively) while examining separately HD patients,
the predictors for the same parameter were ox-LDL and HD duration (beta
= -0.671, t = -4.709, P = 0.000 and beta= -0.349, t = -2.447, P = 0.023,
respectively).
Conclusion: Ox-LDL serum level is inversely correlated to telomerase
activity in PBMC of HD patients. Our study proposes a new consequence of
increased oxidative stress in HD patients: the premature cellular
senescence potentially related to atherosclerosis through LDL oxidation.
Συγγραφείς:
Tsirpanlis, George
Chatzipanagiotou, Stylianos
Boufidou, Fotini
and Kordinas, Vasileios
Zoga, Margarita
Alevyzaki, Fotini and
Stamatelou, Kyriaki
Frangou, Eleni
Savva, Lefkothea and
Nicolaou, Chrysoula
