Τίτλος:
Application of the ELISPOT method for comparative analysis of
interleukin (IL)-6 and IL-10 secretion in peripheral blood of patients
with astroglial tumors
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Glioblastoma, (grade IV astrocytoma), is characterized by rapid growth
and resistance to treatment. Identification of markers of aggressiveness
in this tumor could represent new therapeutic targets. Interleukins
(IL)-6 and IL-10 may be considered as possible candidates, regulating
cell growth, resistance to chemotherapy and angiogenesis. ELISPOT method
provides a useful tool for the determination of the exact cell number of
peripheral lymphocytes secreting a specific cytokine. IL-6 and IL-10
secretion levels were determined using ELISPOT methodology in peripheral
blood mononuclear cells of 18 patients with astrocytic neoplasms (3
grade II and 15 grade IV), in parallel with 18 healthy controls.
Additionally, immunohistochemical expression of these two cytokines was
performed in paraffin-embedded neoplastic tissue in 12 of these
patients. The secretion of IL-6 from peripheral monocytes was
significantly higher in glioma patients compared to controls (P =
0.0003). In addition, IL-10 secretion from peripheral mononuclear and
tumor cells of glioma patients was also higher as compared to healthy
controls (P = 0.0002). Based on immunohistochemical staining, IL-6
expression was localized in tumor cells and macrophages as well as in
areas of large ischemic necrosis, while the major source of IL-10
expression in glioblastomas was the microglia/macrophage cells. It is
suggested that IL-10 contributes to the progression of astrocytomas by
suppressing the patient’s immune response, whereas IL-6 provides an
additional growth advantage. This study demonstrates for the first time
the usefulness of ELISPOT in estimating the secretion of IL-6 and IL-10
from peripheral blood and the correlation of their expression in
neoplastic cells.
Συγγραφείς:
Samaras, Vassilis
Piperi, Christina
Korkolopoulou, Penelope and
Zisakis, Athanasios
Levidou, Georgia
Themistocleous, Marios S.
and Boviatsis, Efstathios I.
Sakas, Damianos E.
Lea, Robert W.
and Kalofoutis, Anastasios
Patsouris, Efstratios
Περιοδικό:
Molecular and Cellular Biochemistry
Λέξεις-κλειδιά:
IL-6; IL-10; astroglial tumors; peripheral blood; neoplastic tissue;
gliomas
DOI:
10.1007/s11010-007-9517-3
