Τίτλος:
Expression of APOBEC family members as regulators of endogenous retroelements and malignant transformation in systemic autoimmunity
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Objective: To explore whether APOBEC family members are involved in the response to inappropriate expression of L1 retroelements in primary Sjögren's syndrome (SS) and systemic lupus erythematosus (SLE), as well as in SS related lymphomagenesis. Methods: Minor salivary glands (MSG) and kidney biopsy (KB) specimens were obtained from 41 SS patients (10 with lymphoma) and 23 patients with SLE, respectively. PBMC and sera were also collected from 73 SLE patients. Full-length L1 transcripts, members of the APOBEC and IFN family were quantitated by real time PCR. Type I IFN activity was assessed in lupus plasma by a cell assay. Results: APOBEC3A was increased in SS MSG, SLE KB and PBMC and correlated with L1. AID and APOBEC3G were particularly overexpressed in MSG tissues derived from SS lymphoma patients. Conclusion: These data reveal a previously unappreciated role of APOBEC family proteins in the pathogenesis of systemic autoimmunity and SS related lymphomagenesis. © 2020
Συγγραφείς:
Mavragani, C.P.
Kirou, K.A.
Nezos, A.
Seshan, S.
Wild, T.
Wahl, S.M.
Moutsopoulos, H.M.
Crow, M.K.
Περιοδικό:
Clinical Immunology Newsletter
Εκδότης:
Academic Press Inc.
Λέξεις-κλειδιά:
activation induced cytidine deaminase; alpha2 interferon; apolipoprotein B messenger RNA editing enzyme catalytic polypeptide 3G; apolipoprotein B mRNA editing enzyme catalytic polypeptide 3A; apolipoprotein B mRNA editing enzyme catalytic polypeptide 3B; apolipoprotein B mRNA editing enzyme catalytic polypeptide like; gamma interferon; messenger RNA; unclassified drug; AICDA (activation-induced cytidine deaminase); APOBEC3A protein, human; cytidine deaminase; interferon; protein, adult; Article; autoimmunity; case control study; cell assay; controlled study; exploratory research; female; gene expression regulation; gene overexpression; genetic association; human; human cell; human tissue; kidney biopsy; kidney tissue; lupus erythematosus nephritis; lymphoma; lymphomagenesis; major clinical study; male; malignant transformation; middle aged; minor saliva gland; mRNA expression level; oncogenesis and malignant transformation; peripheral blood mononuclear cell; plasma; priority journal; protein expression level; protein family; real time polymerase chain reaction; regulatory mechanism; retroposon; serum; Sjoegren syndrome; systemic lupus erythematosus; autoimmunity; cell culture; cell transformation; endogenous retrovirus; genetics; immunology; kidney; metabolism; mononuclear cell; physiology; salivary gland, Autoimmunity; Cell Transformation, Neoplastic; Cells, Cultured; Cytidine Deaminase; Endogenous Retroviruses; Gene Expression Regulation; Humans; Interferons; Kidney; Leukocytes, Mononuclear; Lupus Erythematosus, Systemic; Lymphoma; Proteins; Salivary Glands; Sjogren's Syndrome
DOI:
10.1016/j.clim.2020.108649
