Τίτλος:
Experimental Models of Partial Intestinal Obstruction in Young Mice, Establishment, and Evaluation
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: Partial small bowel obstruction (SBO) is a common, potentially hazardous, surgical entity caused by numerous factors in humans. A number of techniques have been reported as efficient to simulate partial SBO in murine models. However, there is little data concerning their long-term survival. Our study presents a novel technique and evaluates its long-term efficiency compared with other commonly used techniques. Materials and methods: Sixty C57BL/6 mice aged 6 to 8 wk were randomly divided into five intervention groups: ligation, intestinal ring, partial ligation, microclips, and the novel triple suture technique. The ring groups were subdivided into narrow, medium, and wide ring and partial ligation groups were subdivided at 1/3, 1/2, and 2/3 of the lumen. Survival cutoff time was set at 4 wk. Animals were then euthanized and small bowel muscle layer thickness was histopathologically evaluated. Results: None of the animals of the ligation and the ring groups reached the cutoff survival time. The mortality rate of the partial ligation and the microclips groups at the 4-week period were 33.3% and 0%, respectively. However, elimination of the performed intervention was revealed at the time of euthanasia and no alterations of the muscle layer were revealed at histopathology. The “triple suture” group had a survival rate of 90% until euthanasia and the sutures were apparent in all cases. Macroscopic evaluation showed small to mild proximal lumen dilatation in 6 of 10 animals. Histopathological evaluation of the specimens confirmed the partial obstruction. Conclusions: The “triple suture” technique is a new, robust, reliable, and inexpensive technique for experimental long-standing partial SBO, with very low mortality. © 2020 Elsevier Inc.
Συγγραφείς:
Georgopoulos, I.
Mavrigiannaki, E.
Stasinopoulou, S.
Renieris, G.
Nikolakis, G.
Chaniotakis, I.
Tiniakos, D.
Papaconstantinou, I.
Περιοδικό:
Journal of Surgical Research
Εκδότης:
Academic Press Inc.
Λέξεις-κλειδιά:
animal euthanasia; animal experiment; animal model; animal tissue; animal welfare; Article; dilatation; experimental model; female; histopathology; hyperplasia; incision; intestine loop; ligation; long term survival; male; mesentery; mortality rate; mouse; mouse model; muscle hypertrophy; nonhuman; priority journal; small intestine content; small intestine muscle; small intestine obstruction; small intestine wall; smooth muscle cell; surgical technique; survival rate; survival time; suture technique; time of death; adverse event; animal; C57BL mouse; disease model; economics; education; human; intestine mucosa; intestine obstruction; pathology; pathophysiology; randomization; reproducibility; small intestine; surgery, Animals; Disease Models, Animal; Female; Humans; Intestinal Mucosa; Intestinal Obstruction; Intestine, Small; Ligation; Male; Mice; Mice, Inbred C57BL; Random Allocation; Reproducibility of Results; Suture Techniques
DOI:
10.1016/j.jss.2020.03.007
