Τίτλος:
Methylation profile of genes involved in inflammation, in the blood from pregnancies with maternal preeclampsia due to untreated gestational diabetes mellitus
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Purpose: To investigate DNA methylation changes in peripheral blood from patients with gestational diabetes mellitus (GDM) and preeclampsia (PE) due to poorly treated GDM. Methods: Eighteen pregnant women participated in the study: 6 with GDM, 6 with PE, and 6 healthy controls. The promoter methylation status of genes was profiled using the Human Inflammatory Response and Autoimmunity EpiTect Methyl II Signature PCR Array profiles. The results were validated with quantitative real-time polymerase chain reaction (qRT-PCR). Results: Fewer inflammation-related genes were significantly hypomethylated in PE cases compared to healthy subjects than in GDM cases. Some of the examined genes show different methylation patterns between GDM and PE. Conclusions: The epigenetic changes observed in this study indicate that GDM and PE exhibit specific DNA methylation profiles, with possible clinical applications. © 2019, Hellenic Endocrine Society.
Συγγραφείς:
Halvatsiotis, P.
Tsokaki, T.
Chrelias, C.
Kassanos, D.
Domali, E.
Gazouli, M.
Dimitriadis, G.
Kalantaridou, S.
Περιοδικό:
Vitamins and Hormones
Λέξεις-κλειδιά:
activating transcription factor 2; CXCL14 chemokine; CXCL3 chemokine; DNA; epithelial derived neutrophil activating factor 78; granulocyte chemotactic protein 2; interleukin 10 receptor alpha; interleukin 12p35; interleukin 12p40; interleukin 13; interleukin 15; interleukin 17C; interleukin 6 receptor; interleukin 7; transcription factor GATA 3; biological marker; transcriptome, adult; Article; ATF2 gene; CCL25 gene; clinical article; controlled study; CXCL14 gene; CXCL3 gene; CXCL5 gene; CXCL6 gene; diastolic blood pressure; DNA methylation; female; GATA3 gene; gene; gene expression regulation; human; IL10RA gene; IL12A gene; IL12B gene; IL13 gene; IL13RA1 gene; IL15 gene; IL17RA gene; IL4R gene; IL6R gene; IL6ST gene; IL7 gene; IL7C gene; INHA gene; mRNA expression level; preeclampsia; pregnancy diabetes mellitus; systolic blood pressure; blood; blood cell; case control study; complication; disease exacerbation; gene expression profiling; genetics; inflammation; metabolism; microarray analysis; pathology; preeclampsia; pregnancy; pregnancy diabetes mellitus; prognosis; promoter region, Adult; Biomarkers; Blood Cells; Case-Control Studies; Diabetes, Gestational; Disease Progression; DNA Methylation; Female; Gene Expression Profiling; Humans; Inflammation; Microarray Analysis; Pre-Eclampsia; Pregnancy; Prognosis; Promoter Regions, Genetic; Transcriptome
DOI:
10.1007/s42000-019-00111-x
