Τίτλος:
Bioartificial liver attenuates intestinal mucosa injury and gut barrier dysfunction after major hepatectomy: Study in a porcine model
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background The aim of this study was to evaluate whether bioartificial liver support can attenuate gut mucosa injury in a porcine model of posthepatectomy liver dysfunction. Methods Posthepatectomy liver failure was induced in pigs combining major (70%) liver resection and ischemia/reperfusion injury. An ischemic period of 150 minutes was followed by reperfusion for 24 hours. Animals were divided randomly into 2 groups: a control group (n = 6) that received standard critical care and a bioartificial liver support group (Hepat, n = 6) that were subjected to extracorporeal liver support for 6 hours during reperfusion. Intestinal mucosal injury, bacterial translocation, and endotoxin translocation were evaluated in all animals. Intestinal mucosa was also evaluated with markers of oxidative injury and immunohistochemical staining for caspase 3. Results When compared with median values, the control group, animals in the Hepat group had a lesser intestinal mucosal injury score (4.0 [range:2.0-5.0] vs 1.0 [range:1.0-2.0]; P <.01), decreased bacterial translocation in the portal and the systemic circulation at 24 hours of reperfusion (P <.05), and decreased portal and systemic endotoxin levels at 24 hours (P <.05). At 24 hours after reperfusion, mucosal protein carbonyls and malondialdehyde levels were decreased in Hepat animals (0.57 nmol/mg [range:0.32-0.70] vs 0.33 nmol/mg [range:0.03-0.53] and 3.85 nmol/mg [range:3.01-6.43] vs 3.27 nmol/mg [range:1.46-3.55], respectively; P <.05). There was no difference in tissue caspase staining. Conclusion Bioartificial liver support seems to attenuate intestinal mucosal injury and gut barrier dysfunction after major hepatectomy. © 2016 Elsevier Inc. All rights reserved.
Συγγραφείς:
Nastos, C.
Kalimeris, K.
Papoutsidakis, N.
Defterevos, G.
Pafiti, A.
Kalogeropoulou, E.
Zerva, L.
Nomikos, T.
Papalois, A.
Kostopanagiotou, G.
Smyrniotis, V.
Arkadopoulos, N.
Περιοδικό:
Otolaryngology - Head and Neck Surgery (United States)
Εκδότης:
Mosby Year Book Inc
Λέξεις-κλειδιά:
antioxidant; biological marker; carbonyl derivative; caspase 3; Escherichia coli endotoxin; malonaldehyde; protein carbonyl; unclassified drug; endotoxin, animal cell; animal experiment; animal model; animal tissue; apoptosis; Article; bacterial translocation; bioartificial liver; controlled study; endotoxin-induced liver injury; enteropathy; extracorporeal circulation; female; gut barrier dysfunction; hemodynamics; histopathology; immunohistochemistry; intensive care; intestinal mucosa injury; intestine injury; intestine mucosa; intestine mucosa permeability; liver ischemia; liver resection; major surgery; nonhuman; oxidative stress; porcine model; portal vein blood flow; priority journal; protein carbonylation; reperfusion injury; systemic circulation; adverse effects; animal; artificial liver; disease model; injuries; liver failure; pig; reperfusion injury, Animals; Bacterial Translocation; Disease Models, Animal; Endotoxins; Female; Hepatectomy; Intestinal Mucosa; Liver Failure; Liver, Artificial; Oxidative Stress; Reperfusion Injury; Swine
DOI:
10.1016/j.surg.2015.12.018
