Τίτλος:
Associations of combined polymorphisms of the platelet membrane glycoproteins Ia and IIIa and the platelet-endothelial cell adhesion molecule-1 and P-Selectin genes with IVF implantation failures
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The aim of the study was to investigate the combined impact of the genetic heterogeneity of the glycoproteins Ia (GpIa) and IIIa (GpIIIa) and the platelet-endothelial cell adhesion molecule-1 (PECAM-1) and P-Selectin genes on IVF embryo transfer implantation failures (IVF-ET failures). Sixty nulligravida women with previous IVF-ET failures and 60 fertile controls were genotyped for the GpIa-C807T, GpIIIa-PlA1/PA2, PECAM-1-C373G (Leu125Val) and P-Selectin-A37674C (Thr715Pro) polymorphisms by pyrosequencing. Compared with wild-type combined homozygotes, carriers of combinations of risk alleles in two gene loci were at significantly increased risk for IVF-ET failure, whereas carriers of the combination of GpIa-807T, GpIIIa-PlA2 and PECAM-1-373G alleles had OR = 52.50 (95%CI: 4.05–680.95, p <.001). The area under the receiver-operating characteristic curve (AUC) based on the number of polymorphisms and the number of risk alleles per subject was 75.4% (95%CI: 66.7%–82.8%, p <.001) and 72.5% (95%CI: 63.6%–80.3%, p <.001), respectively. The OR per polymorphism and risk allele increase was 4.26 (95%CI: 2.15–8.41, p <.001) and 2.85 (95%CI: 1.71–4.76, p <.001), respectively. The above associations were more robust among younger women. The combined analysis of these polymorphisms revealed strong association of combined carriers with IVF-ET failures especially for younger women and provided a genetic risk score with good diagnostic accuracy in the prediction of IVF-ET failures. © 2017 Informa UK Limited, trading as Taylor & Francis Group.
Συγγραφείς:
Vlachadis, N.
Tsamadias, V.
Vrachnis, N.
Kaparos, G.
Vitoratos, N.
Kouskouni, E.
Economou, E.
Περιοδικό:
JOURNAL OF OBSTETRICS AND GYNAECOLOGY
Εκδότης:
Taylor and Francis Ltd.
Λέξεις-κλειδιά:
alpha2 integrin; CD31 antigen; glycoprotein IIIa; PADGEM protein; alpha2 integrin; beta3 integrin; biological marker; CD31 antigen; PADGEM protein, adult; allele; Article; controlled study; embryo transfer; female; gene locus; genetic association; genetic heterogeneity; genetic risk; genotype; human; major clinical study; nidation; nulligravida; priority journal; protein polymorphism; pyrosequencing; secondary analysis; age; area under the curve; blood; case control study; genetics; heterozygote; in vitro fertilization; nidation; risk; sensitivity and specificity; single nucleotide polymorphism; treatment failure, Adult; Age Factors; Antigens, CD31; Area Under Curve; Biomarkers; Case-Control Studies; Embryo Implantation; Female; Fertilization in Vitro; Heterozygote; Humans; Integrin alpha2; Integrin beta3; P-Selectin; Polymorphism, Single Nucleotide; Risk; Sensitivity and Specificity; Treatment Failure
DOI:
10.1080/01443615.2016.1256978
