Τίτλος:
Comparison of erythropoietin and sildenafil protective role against ischemia/reperfusion injury of the testis in adult rats
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Purpose: Tissue damage in testicular torsion/detorsion is caused not only by the ischemia, but also by the ischemia/reperfusion injury after detorsion. Erythropoietin and sildenafil are considered to protect against ischemia/reperfusion injury. Here, we studied and compared their actions in testicular torsion/detorsion in adult rats. Methods: Twenty-two adult male Wistar Albino rats were divided into four groups. Rats in group A (n = 5) were sham operated. Rats in group B (n = 5), group C (n = 6) and group D (n = 6) underwent torsion of the right testis and detorsion after 90 min. No pharmaceutical intervention was performed in group B. Erythropoietin (1,000 IU/kg) and sildenafil (0.7 mg/kg) were injected intraperitoneally in groups C and D, respectively, after 60 min of torsion. All animals were killed 24 h after detorsion, and their right testis was extracted, placed into 10 % formalin solution and sent for histopathological examination. The histological changes in the testes were scored according to the four-point grading system proposed by Cosentino et al. Results: All rats in group A had normal testicular architecture (grade 1). The untreated group B had a mean grade of 3.81 (range 3.65-4). The treated groups C (mean grade 3.24; range 3.05-3.45) and D (2.69, range 2.4-2.9) presented statistically significant better results (lower grades) compared with the untreated group B. Group D had significantly better results (lower grades) than group C. Conclusions: The intraperitoneal injection of erythropoietin and sildenafil protects against ischemia/reperfusion injury after testicular torsion and detorsion. Sildenafil may have a stronger action than erythropoietin at the doses used in this study. © 2013 Springer Science+Business Media Dordrecht.
Συγγραφείς:
Zavras, N.
Kostakis, I.D.
Sakellariou, S.
Damaskos, C.
Roupakas, E.
Tsagkari, E.
Spartalis, E.
Velaoras, K.
Dontas, I.A.
Karatzas, T.
Περιοδικό:
International Urology and Nephrology
Εκδότης:
Kluwer Academic Publishers
Λέξεις-κλειδιά:
novel erythropoiesis stimulating protein; sildenafil; erythropoietin; phosphodiesterase V inhibitor; piperazine derivative; purine derivative; sildenafil; sulfone, adult; animal experiment; animal model; animal tissue; article; controlled study; histopathology; male; nonhuman; rat; reperfusion injury; testis detorsion; testis disease; testis injury; testis torsion; treatment outcome; animal; comparative study; complication; intraperitoneal drug administration; pathology; reperfusion injury; testis; testis torsion; vascularization; Wistar rat, Animals; Erythropoietin; Injections, Intraperitoneal; Male; Phosphodiesterase 5 Inhibitors; Piperazines; Purines; Rats, Wistar; Reperfusion Injury; Spermatic Cord Torsion; Sulfones; Testis
DOI:
10.1007/s11255-013-0569-x
