Τίτλος:
Experimental replacement of pig trachea with novel bioprosthesis from harp Seal
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Tracheal replacement has been a challenging problem for thoracic surgeons for over half of a century. We evaluated the in-vivo performance of a new tracheal bioprosthesis derived from Harp seal (Phoca groelandica) trachea that was fixed and preserved in 0.625% buffered glutaraldehyde solution for 3 months. Ten young male pigs weighing 27-32 kg (mean, 28.7 kg) underwent replacement of a tracheal segment with this new bioprosthesis. The length of replaced trachea was 1.8-2.4 cm (mean, 2.17 cm), representing 2-3 cartilage rings. All pigs survived the operation uneventfully. No immunosuppression drugs were used. The pigs eventually developed dyspnea and were euthanized on postoperative day 17-39 (mean, 30.8 days). Macroscopic and histological analysis showed an intact bioprosthesis but near-total occlusion of the native trachea by a ring of inflammatory infiltration at the site of distal anastomosis. More experiments involving a different concentration of the preservation agent, different management, and perhaps the use of bioengineering techniques are needed to improve the performance of this novel bioprosthesis. © 2010 SAGE Publications.
Συγγραφείς:
Agathos, E.A.
Tomos, P.
Lachanas, E.
Gakiopoulou, H.
Pantopoulou, A.
Perrea, D.
Περιοδικό:
Asian Cardiovascular and Thoracic Annals
Λέξεις-κλειδιά:
glutaraldehyde; fixative, animal experiment; article; bioprosthesis; controlled study; dyspnea; in vivo study; male; nonhuman; seal; swine; trachea; trachea surgery; animal; evaluation; foreign body reaction; implantation; instrumentation; materials testing; methodology; pathology; Phoca; prosthesis; thorax surgery; time; tissue fixation; trachea; trachea stenosis; transplantation, Animals; Bioprosthesis; Fixatives; Foreign-Body Reaction; Glutaral; Male; Materials Testing; Prosthesis Design; Prosthesis Implantation; Seals, Earless; Swine; Thoracic Surgical Procedures; Time Factors; Tissue Fixation; Trachea; Tracheal Stenosis
DOI:
10.1177/0218492310387448
