Τίτλος:
Apoptotic and inflammation markers in oral mucositis in head and neck cancer patients receiving radiotherapy: Preliminary report
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Goal of work: The aim of this study was to investigate the expression of pro-apoptotic protein p53 and anti-apoptotic proteins BCl-2 and MCl-1, as well as the expression of pro-inflammatory cytokines tumor necrosis factor (TNF) and interleukin-1beta (IL-1β) in patients developing mucositis during radiotherapy for head and neck cancer. Materials and methods: Thirty-five patients receiving radiotherapy for head/neck cancer were included in this study. Patients were examined before radiotherapy. Oral mucositis was recorded weekly during radiotherapy. Cytologic smears from the oral cavity were taken with a brush. Immunocytochemical staining was performed by the use of p53, BCl-2, MCl-1 TNF and IL-1β monoclonal antibodies. Main results: P53 was expressed in 1 of 15 smears before the initiation of radiotherapy (6.5%) compared to 3 of 7 smears from patients with grade III mucositis (43%) during radiotherapy. BCl-2 was expressed in 15 of 15 smears before radiotherapy (100%) and in three of seven patients with grade III mucositis (43%) during radiotherapy. MCl-1 was expressed in 10 of 14 samples before radiotherapy (71.5%) and in two of seven patients with grade III (28.5%) mucositis during radiotherapy. TNF was expressed in 9 of 14 patients before radiotherapy (64%) and in six of seven patients with grade III mucositis during radiotherapy (86%). IL-1β was detected in 7 of 14 patients before radiotherapy (50%) compared to 6 of 7 patients with grade III mucositis during radiotherapy (86%). Conclusion: Our preliminary results indicate an induction of apoptosis and inflammation in the oral mucosa in patients developing mucositis during radiotherapy for head/neck cancer. © 2007 Springer-Verlag.
Συγγραφείς:
Xanthinaki, A.
Nicolatou-Galitis, O.
Athanassiadou, P.
Gonidi, M.
Kouloulias, V.
Sotiropoulou-Lontou, A.
Pissakas, G.
Kyprianou, K.
Kouvaris, J.
Patsouris, E.
Περιοδικό:
Supportive Care in Cancer
Λέξεις-κλειδιά:
interleukin 1beta; monoclonal antibody; protein bcl 2; protein mcl 1; protein p53; tumor marker; tumor necrosis factor alpha, adult; aged; apoptosis; article; cancer patient; cancer radiotherapy; clinical article; disease marker; disease severity; female; head and neck cancer; human; human tissue; immunocytochemistry; male; mouth cavity; mouth inflammation; mucosa inflammation; priority journal; protein determination; protein expression; smear, Adult; Aged; Aged, 80 and over; Apoptosis; Female; Head and Neck Neoplasms; Humans; Immunohistochemistry; Inflammation; Interleukin-1beta; Karnofsky Performance Status; Male; Middle Aged; Pilot Projects; Proto-Oncogene Proteins c-bcl-2; Radiotherapy; Risk Factors; Severity of Illness Index; Stomatitis; Tumor Markers, Biological; Tumor Necrosis Factor-alpha
DOI:
10.1007/s00520-007-0379-8
