Τίτλος:
Increased apoptosis in the alveolar microenvironment of the healthy
human lung
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background. Apoptosis represents a physiological clearance mechanism in
human tissues. The role of apoptosis has not been examined in normal
lung cell populations, such as alveolar macrophages and
polymorphonuclear cells. What is the percentage, as well as the role, of
apoptosis in the alveolar microenvironment of the healthy human lung?
Patients and methods. Bronchoalveolar lavage was obtained from 21
volunteers without lung disease. The specimens were analyzed using:
Annexin V binding, DNA laddering, light microscopy and
immunohistochemistry for bcl-2 expression.
Results. Apoptosis of the total bronchoalveolar lavage cell population
was 51.2%. Both alveolar macrophages and polymorphonuclear cells had a
high apoptotic rate (62.1% and 48.3%, respectively) as determined by
Annexin V binding. These findings were further confirmed using
morphological criteria for apoptosis and gel electrophoresis for DNA
fragmentation. In the majority of the individuals examined, (8 out of
21), the bcl-2 gene was expressed in the lymphocyte population mainly.
Conclusions. The percentage of apoptosis in lung cells of healthy humans
is high. Apoptosis plays a key role in normal lung cell death. It
appears to be the mechanism that opposes cell proliferation by
eliminating, aged or damaged cells thus facilitating the process of lung
remodeling. (C) 2008 Elsevier Inc. All rights reserved.
Συγγραφείς:
Liacos, Christine
Konstadoulakis, Manousos M.
Economou, Venetia
and Katsaragakis, Stylianos
Chatzigianni, Emmy
Georgiadis,
George G.
Prekates, Athanasios
Karampinis, Andreas
Bramis,
John
Περιοδικό:
Journal of Surgical Research
Εκδότης:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Λέξεις-κλειδιά:
bronchoalveolar lavage; alveolar macrophages; polymorphonuclear cells;
Annexin V; DNA ladder
DOI:
10.1016/j.jss.2006.06.037
