Τίτλος:
Long-term primary cultures of human adult atrial cardiac myocytes: Cell
viability, structural properties and BNP secretion in vitro
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: Human adult cardiomyocytes (CM) have been used in short-term
cultures for in vitro studies of the adult myocardium. However, little
information is available regarding human adult CMs cultured for long
term (>2 weeks).
Methods: Human adult CMs were isolated from atrial specimens of 43
patients undergoing cardiopulmonary bypass surgery. Cell viability,
cytoskeletal properties, intercellular junctional mediators and
responsiveness to extracellular stimuli were monitored in CM cultures
for 8 weeks.
Results: Absolute numbers of CMs decreased through the first 2 weeks,
with substantially lower rates of cell loss thereafter. Apoptosis
predominated over necrosis as the principal mode of cell death,
affecting 4.1 +/- 1.6% of freshly dissociated cells, that declined in
culture (3.6 +/- 1.0% week 1, 1.3 +/- 0.5% week 2). CMs maintained
rod-shaped morphology and cross-striated expression pattern of
sarcomeric proteins desmin and beta-myosin heavy chain for the first 4
weeks. Levels of desmin remained stable on first 3 weeks, but declined
thereafter. CMs expressed cardiac-specific adherence molecule N-cadherin
throughout the culture duration, indicating conserved contractile
potential. CMs remained functional early in culture, as indicated by BNP
secretion, with maximal levels on 1st week that declined gradually by
week 4. Cell responsiveness to metabolic stresses (serum deprivation)
was detected, inducing an early (6 h) 1.8-fold increase in levels of
BNP.
Conclusion: Long-term cultured human adult CMs maintain morphological
integrity, adult-type cytoskeletal protein expression, cell-cell
communication potential and functionality for 3-4 weeks in vitro. (C)
2007 Elsevier Ireland Ltd. All rights reserved.
Συγγραφείς:
Bistola, Vasiliki
Nikolopoulou, Marilena
Derventzi, Anastasia
and Kataki, Agapi
Sfyras, Nikolaos
Nikou, Niki
Toutouza,
Marina
Toutouzas, Pavlos
Stefanadis, Christodoulos and
Konstadoulakis, Manousos M.
Περιοδικό:
International Journal of Cardiology
Εκδότης:
Elsevier Ireland Ltd
Λέξεις-κλειδιά:
Cardiac myocytes; Culture; Apoptosis; Cytoskeleton; b-type natriuretic
peptide; Adhesion molecules
DOI:
10.1016/j.ijcard.2007.10.058
