Τίτλος:
Early hCG addition to rFSH for ovarian stimulation in IVF provides
better results and the cDNA copies of the hCG receptor may be an
indicator of successful stimulation
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
A simple, safe and cost-effective treatment protocol in ovarian
stimulation is of great importance in IVF practice, especially in the
case of previous unsuccessful attempts. hCG has been used as a
substitute of LH because of the degree of homology between the two
hormones. The main aim of this prospective randomized study was to
determine, for the first time, whether low dose hCG added to rFSH for
ovarian stimulation could produce better results compared to the
addition of rLH in women entering IVF-ET, especially in those women that
had previous IVF failures. An additional aim was to find an indicator
that would allow us to follow-up ovarian stimulation and, possibly,
modify it in order to achieve a better IVF outcome; and that indicator
may be the cDNA copies of the LH/hCG receptor. Group A patients (n = 58)
were administered hCG and Group B rLH (n = 56) in addition to rFSH in
the first days of ovarian stimulation. The number of follicles and
oocytes and, most importantly, implantation and pregnancy rates were
shown to be statistically significantly higher in the hCG group. This
study has also determined, for the first time to our best knowledge,
m-RNA for LH/hCG receptors in the lymphocytes of peripheral blood 40 h
before ovum pick-up. cDNA levels of the hCG receptor after ovarian
stimulation were significantly higher among women receiving hCG compared
to those receiving LH. In addition, higher levels were encountered among
women with pregnancy compared to those without, although this was not
statistically significant due to the small number of pregnancies. It
seems that hCG permits a highly effective and more stable occupancy of
rLH/hCG receptors and gives more follicles and more oocytes. The
determination of cDNA copies could be, in the future, a marker during
ovulation induction protocols and of course a predictor for the outcome
of ART in the special subgroup of patients with previous failures.
Συγγραφείς:
Drakakis, Peter
Loutradis, Dimitris
Beloukas, Apostolos and
Sypsa, Vana
Anastasiadou, Vasiliki
Kalofolias, George and
Arabatzi, Helen
Kiapekou, Erasmia
Stefanidis, Konstantinos and
Paraskevis, Dimitris
Makrigiannakis, Antonis
Hatzakis, Angelos
and Antsaklis, Aris
Περιοδικό:
Reproductive Biology and Endocrinology
DOI:
10.1186/1477-7827-7-110
