Τίτλος:
SETTING A RATIONAL FRAMEWORK FOR EXPERIMENTAL DESIGN AND ANALYSIS OF
HIGH-THROUGHPUT DNA MICROARRAY EXPERIMENTS AND DATA
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Microarray experimentation has been widely used for screening and high
throughput discovery of mechanisms underlying biological systems.
However, there are many factors that need to be taken into account for
an effective microarray experimental design. Such factors are the
abundance of starting material (RNA), number of replicates or
experimental cost. The present work proposes rational strategies for
microarray experimental design, with the scope to minimize signal
variance and improve precision, with the minimum amount of technical
replicates needed, thus alleviating the burden of biological material
collection and the cost of the experiment. In this sense, alternative
microarray design strategies are tested (direct comparisons, all paired
comparisons, indirect comparisons through the implementation of various
loop strategies), targeting to minimize the wet lab experimental effort
needed, for the more precise interpretation of the biological question
investigated. In this work we propose a methodological approach for the
computational implementation of comparisons between different microarray
experimental samples which are not co-hybridized together. Moreover,
this approach is tested in-silico, on a showcase of a simple “loop”
experimental design, including 3 biological triplicates. The proposed
methodological approach introduces a robust method for calculating and
comparing gene expression profiles between sets of microarray
experiments by using the minimum number of experiments on one hand and
getting robust results on the other. Further extension of this work
could form a rational operational computational framework for the design
of microarray or more general high-throughput biological experiments.
Συγγραφείς:
Lambrou, George I.
Chatziioannou, Aristotelis
Sifakis, Emmanouil
and Prentza, Andriana
Koutsouris, Dimitris
Koultouki, Eleftheria
and Tzortzatou-Stathopoulou, Fotini
Εκδότης:
WORLD SCIENTIFIC PUBL CO PTE LTD
Τίτλος συνεδρίου:
ADVANCED TOPICS IN SCATTERING THEORY AND BIOMEDICAL ENGINEERING
DOI:
10.1142/9789814322034_0013
