Τίτλος:
Analysis of Secreted Proteins for the Study of Bladder Cancer Cell
Aggressiveness
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Secreted proteins play a key role in cell signaling, communication, and
migration. We recently described the development of an aggressive
variant (T24M) of the bladder cancer cell line T24. Using this cell line
model, the objective of our work was the identification of secreted
proteins involved in the acquisition of the aggressive phenotype. Using
in vitro assays, we demonstrate that conditioned media of the T24M cells
promote motility of the parental less aggressive T24 cells. Proteomic
analysis of cell culture conditioned media by the use of 2-dimensional
gel electrophoresis coupled to MALDI TOF MS and LC-MS approaches
resulted in enrichment and detection of multiple classical extracellular
and secreted proteins such as fibronectin, cystatin, fibrillin, fibulin,
interleukin 6, etc. Comparison of the secretome of the T24 and T24M
cells indicated differences in proteins with potential involvement in
the mechanisms of cell aggressiveness including SPARC, tPA, and
clusterin. These findings were further confirmed by Western blot
analysis. In the case of SPARC, further studies involving transwell
assays indicated that blockage of the protein in the presence of
SPARC-specific Abs results in decreased cell motility. Collectively, our
study provides a 2DE-based comprehensive analysis of bladder cancer cell
secretome. The results indicate various secreted proteins with potential
involvement in bladder cancer cell aggressiveness and more specifically
provide initial evidence for special role of SPARC in bladder cancer
cell motility and invasiveness.
Συγγραφείς:
Makridalds, Manousos
Roubelaids, Maria G.
Bitsika, Vasiliki and
Dimuccio, Veronica
Samiotaki, Martina
Kossida, Sophia and
Panayotou, George
Coleman, Jonathan
Candiano, Giovanni and
Anagnou, Nikolaos P.
Vlahou, Antonia
Περιοδικό:
Journal of Proteome Research
Εκδότης:
AMER CHEMICAL SOC
Λέξεις-κλειδιά:
bladder cancer; proteomics; T24; secretome; SPARC; 2DE
