Multicenter Study for Defining the Breakpoint for Rifampin Resistance in Neisseria meningitidis by rpoB Sequencing

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Μονάδα:
Ερευνητικό υλικό ΕΚΠΑ
Τίτλος:
Multicenter Study for Defining the Breakpoint for Rifampin Resistance in
Neisseria meningitidis by rpoB Sequencing
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Identification of clinical isolates of Neisseria meningitidis that are
resistant to rifampin is important to avoid prophylaxis failure in
contacts of patients, but it is hindered by the absence of a breakpoint
for resistance, despite many efforts toward standardization. We examined
a large number (n = 392) of clinical meningococcal isolates, spanning 25
years (1984 to 2009), that were collected in 11 European countries,
Argentina, and the Central African Republic. The collection comprises
all clinical isolates with MICs of >= 0.25 mg/liter (n = 161) received
by the national reference laboratories for meningococci in the
participating countries. Representative isolates displaying rifampin
MICs of <0.25 mg/liter were also examined (n = 231). Typing of isolates
was performed, and a 660-bp DNA fragment of the rpoB gene was sequenced.
Sequences differing by at least one nucleotide were defined as unique
rpoB alleles. The geometric mean of the MICs was calculated for isolates
displaying the same allele. The clinical isolates displaying rifampin
MICs of >1 mg/liter possessed rpoB alleles with nonsynonymous mutations
at four critical amino acid residues, D542, H552, S548, and S557, that
were absent in the alleles found in all isolates with MICs of <= 1
mg/liter. Rifampin-susceptible isolates could be defined as those with
MICs of <= 1 mg/liter. The rpoB allele sequence and isolate data have
been incorporated into the PubMLST Neisseria database
(http://pubmlst.org/neisseria/). The rifampin-resistant isolates
belonged to diverse genetic lineages and were associated with lower
levels of bacteremia and inflammatory cytokines in mice. This biological
cost may explain the lack of clonal expansion of these isolates.
Έτος δημοσίευσης:
2010
Συγγραφείς:
Taha, Muhamed-Kheir
Hedberg, Sara Thulin
Szatanik, Marek and
Hong, Eva
Ruckly, Corinne
Abad, Raquel
Bertrand, Sophie and
Carion, Francoise
Claus, Heike
Corso, Alejandra
Enriquez,
Rocio
Heuberger, Sigrid
Hryniewicz, Waleria
Jolley, Keith A.
and Kriz, Paula
Mollerach, Marta
Musilek, Martin
Neri,
Arianna
Olcen, Per
Pana, Marina
Skoczynska, Anna and
Sorhouet Pereira, Cecilia
Stefanelli, Paola
Tzanakaki, Georgina
and Unemo, Magnus
Vazquez, Julio A.
Vogel, Ulrich
Wasko,
Izabela
Περιοδικό:
Antimicrobial Agents and Chemotherapy
Εκδότης:
AMER SOC MICROBIOLOGY
Τόμος:
54
Αριθμός / τεύχος:
9
Σελίδες:
3651-3658
Επίσημο URL (Εκδότης):
DOI:
10.1128/AAC.00315-10
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