Περίληψη:
Objective: To evaluate the impact of two different luteal phase support
protocols on gene expression of extracellular matrix (ECM) proteins and
adhesion molecules in the human endometrium.
Design: Eighty-four ECM protein and adhesion molecule genes were
analyzed using array-based reverse-transcription polymerase chain
reaction.
Setting: Academic hospital.
Patient(s): Nine oocyte donors.
Intervention(s): Endometrial biopsies were obtained on the day of oocyte
retrieval (group I) and 3-5 days later (group II) after randomization
into 3 groups. Group IIa had no luteal phase support, group IIb had
luteal support with micronized progesterone, and group IIc had luteal
support with progesterone plus 17 beta-estradiol.
Main Outcome Measure(s): Gene expression profiles in relation to
different types of luteal phase support protocols.
Result(s): Compared with the day of retrieval (group I), 24 genes were
significantly up-regulated (4 in group IIa, 14 in group IIb, 22 in group
IIc) and 14 genes were down-regulated (5 in group IIa, 2 in group IIb,
10 in group IIc) on day 3-5 (P < .05). In the luteal support group,
up-regulation occurred predominantly in genes encoding for matrix
metallopeptidases (MMP10, MMP3, MMP9), integrins (ITGA3, ITGA5, ITGB3,
ITGB4), and laminin (LAMB3). In contrast, the most significant
suppression was documented in genes encoding for catenin-D2,
collagen-11A1, and the laminins (LAMA1 and LAMA3). Significant changes
between groups IIb and IIc were also observed in 9 genes.
Conclusion(s): Luteal phase support following controlled ovarian
stimulation has a profound impact on the ECM pathway targeted genes.
(Fertil Steril (R) 2010;94:2264-71. (C) 2010 by American Society for
Reproductive Medicine.)
Συγγραφείς:
Zhao, Yulian
Garcia, Jairo
Kolp, Lisa
Cheadle, Christopher
and Rodriguez, Annabelle
Vlahos, Nikos F.
