Τίτλος:
Adiponectin levels and expression of adiponectin receptors in isolated
monocytes from overweight patients with coronary artery disease
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: Adiponectin has insulin-sensitizing and anti-atherosclerotic
effects, partly mediated through its action on monocytes. We aimed to
determine adiponectin levels and expression of its receptors (AdipoR1
and AdipoR2) in peripheral monocytes from overweight and obese patients
with coronary artery disease (CAD).
Methods: Fifty-five overweight/obese patients, suspected for CAD,
underwent coronary angiography: 31 were classified as CAD patients
(stenosis >= 50% in at least one main vessel) and 24 as nonCAD.
Quantitative RT-PCR and flow cytometry were used for determining mRNA
and protein surface expression of adiponectin receptors in peripheral
monocytes. A high sensitivity multiplex assay (xMAP technology) was used
for the determination of plasma adiponectin and interleukin-10 (IL-10)
secreted levels.
Results: Plasma adiponectin levels were decreased in CAD compared to
nonCAD patients (10.9 +/- 3.1 vs. 13.8 +/- 5.8 mu g/ml respectively, p =
0.033). In multivariable analysis, Matsuda index was the sole
independent determinant of adiponectin levels. AdipoR1 and AdipoR2
protein levels were decreased in monocytes from CAD compared to nonCAD
patients (59.5 +/- 24.9 vs. 80 +/- 46 and 70.7 +/- 39 vs. 95.6 +/- 47.8
Mean Fluorescence Intensity Arbitrary Units respectively, p < 0.05). No
significant differences were observed concerning the mRNA levels of the
adiponectin receptors between CAD and nonCAD patients. AdipoR2 protein
levels were positively correlated with plasma adiponectin and Matsuda
index (r = 0.36 and 0.31 respectively, p < 0.05 for both). Furthermore,
basal as well as adiponectin-induced IL-10 release was reduced in
monocyte-derived macrophages from CAD compared to nonCAD subjects.
Conclusions: Overweight patients with CAD compared to those without CAD,
had decreased plasma adiponectin levels, as well as decreased surface
expression of adiponectin receptors in peripheral monocytes. This fact
together with the reduced adiponectin-induced IL-10 secretion from CAD
macrophages could explain to a certain extent, an impaired
atheroprotective action of adiponectin.
Συγγραφείς:
Kollias, Anastasios
Tsiotra, Panayoula C.
Ikonomidis, Ignatios
and Maratou, Eirini
Mitrou, Panayota
Kyriazi, Erifyli and
Boutati, Eleni
Lekakis, John
Economopoulos, Theofanis and
Kremastinos, Dimitrios T.
Dimitriadis, George
Raptis, Sotirios
A.
Περιοδικό:
Cardiovascular Diabetology
DOI:
10.1186/1475-2840-10-14
