Περίληψη:
Human mesenchymal progenitor cells (MPCs) are considered to be of great
promise for use in tissue repair and regenerative medicine. MPCs
represent multipotent adherent cells, able to give rise to multiple
mesenchymal lineages such as osteoblasts, adipocytes or chondrocytes.
Recently, we identified and characterized human second trimester
amniotic fluid (AF) as a novel source of MPCs. Herein, we found that
early colonies of AF-MPCs consisted of two morphologically distinct
adherent cell types, termed as spindle-shaped (SS) and round-shaped
(RS). A detailed analysis of these two populations showed that
SS-AF-MPCs expressed CD90 antigen in a higher level and exhibited a
greater proliferation and differentiation potential. To characterize
better the molecular identity of these two populations, we have
generated a comparative proteomic map of SS-AF-MPCs and RS-AF-MPCs,
identifying 25 differentially expressed proteins and 10 proteins
uniquely expressed in RS-AF-MPCs. Furthermore, SS-AF-MPCs exhibited
significantly higher migration ability on extracellular matrices, such
as fibronectin and laminin in vitro, compared to RS-AF-MPCs and thus we
further evaluated SS-AF-MPCs for potential use as therapeutic tools in
vivo. Therefore, we tested whether GFP-lentiviral transduced SS-AF-MPCs
retained their stem cell identity, proliferation and differentiation
potential. GFP-SS-AF-MPCs were then successfully delivered into
immunosuppressed mice, distributed in different tissues and survived
longterm in vivo. In summary, these results demonstrated that AF-MPCs
consisted of at least two different MPC populations. In addition,
SS-AF-MPCs, isolated based on their colony morphology and CD90
expression, represented the only MPC population that can be expanded
easily in culture and used as an efficient tool for future in vivo
therapeutic applications.
Συγγραφείς:
Roubelakis, Maria G.
Bitsika, Vasiliki
Zagoura, Dimitra and
Trohatou, Ourania
Pappa, Kalliopi I.
Makridakis, Manousos and
Antsaklis, Aristidis
Vlahou, Antonia
Anagnou, Nicholas P.
