Τίτλος:
LH receptor gene expression in cumulus cells in women entering an ART
program
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Luteinizing hormone (LH) exerts its actions through its receptor (LHR),
which is mainly expressed in theca cells and to a lesser extent in
oocytes, granulosa and cumulus cells. The aim of the present study was
the investigation of a possible correlation between LHR gene and LHR
splice variants expression in cumulus cells and ovarian response as well
as ART outcome.
Forty patients undergoing ICSI treatment for male factor infertility
underwent a long luteal GnRH-agonist downregulation protocol with a
fixed 5-day rLH pre-treatment prior to rFSH stimulation and samples of
cumulus cells were collected on the day of egg collection. RNA
extraction and cDNA preparation was followed by LHR gene expression
investigation through real-time PCR. Furthermore, cumulus cells were
investigated for the detection of LHR splice variants using reverse
transcription PCR.
Concerning LHR expression in cumulus cells, a statistically significant
negative association was observed with the duration of ovarian
stimulation (odds ratio = 0.23, = 0.012). Interestingly, 6 over 7 women
who fell pregnant expressed at least two specific types of LHR splice
variants (735 bp, 621 bp), while only 1 out of 19 women that did not
express any splice variant achieved a pregnancy.
Consequently, the present study provide a step towards a new role of LHR
gene expression profiling as a biomarker in the prediction of ovarian
response at least in terms of duration of stimulation and also a
tentative role of LHR splice variants expression in the prediction of
pregnancy success.
Συγγραφείς:
Papamentzelopoulou, Myrto
Mavrogianni, Despina
Partsinevelos,
George A.
Marinopoulos, Spyros
Dinopoulou, Vasiliki and
Theofanakis, Charalampos
Anagnostou, Elli
Loutradis, Dimitris
Περιοδικό:
Journal of Assisted Reproduction and Genetics
Εκδότης:
SPRINGER/PLENUM PUBLISHERS
Λέξεις-κλειδιά:
LH receptor; Splice variants; Cumulus cells; Ovarian stimulation; ICSI;
LH-priming
DOI:
10.1007/s10815-012-9729-7
