Τίτλος:
Human kallikrein-related peptidase 12 (KLK12) splice variants expression
in breast cancer and their clinical impact
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Kallikrein-related peptidases (KLKs) are a group of 15 serine proteases,
hormonally regulated, and localized on chromosome 19q13.4. Alternative
splicing is a process that plays significant role in the development,
physiology, and different diseases, like cancer. Kallikrein family
numbers more than 82 alternative transcripts. Understanding the role
that those gene transcripts play in various cancer types, could lead to
the discovery of diagnostic markers or drug targets. The present study
was designed to analyze the expression profile of the splice variants of
kallikrein-related peptidase 12 (KLK12) in breast cancer patients and to
evaluate their clinical significance. KLK12 splice variants (KLK12sv3
and KLK12sv1/KLK12sv2) were examined in 69 tissue samples of breast
cancer using quantitative real-time PCR as well as semi-quantitative
PCR. Relative quantitative expression of KLK12 was statistically
associated to clinicopathological parameters. From the splice variants
examined, statistical associations with clinicopathological parameters
were obtained only from KLK12sv3 variant. KLK12sv3 is more frequently
expressed in tumors of lower grade (p = 0.040), early patient TNM stage
(p = 0.024), and smaller tumor size (p = 0.023). Positive KLK12sv3
expression is associated with longer patient disease-free survival (DFS)
(p = 0.042) and higher progesterone receptor concentration (p = 0.008).
KLK12sv1/KLK12sv2 expression is statistically associated with KLK12sv3
expression (p = 0.001). KLK12sv3 can be regarded as a marker of good
prognosis in breast cancer.
Συγγραφείς:
Talieri, Maroulio
Devetzi, Marina
Scorilas, Andreas
Pappa,
Eleana
Tsapralis, Nikolaos
Missitzis, Ioannis
Ardavanis,
Alexandros
Εκδότης:
SAGE Publications Ltd
Λέξεις-κλειδιά:
Breast cancer; Cell lines; Kallikrein-related peptidase 12; KLK12;
Splice variants
DOI:
10.1007/s13277-012-0347-x
