Τίτλος:
Identification of Medically Relevant Species of Arthroconidial Yeasts by
Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass
Spectrometry
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Matrix-assisted laser desorption ionization-time of flight mass
spectrometry (MALDI-TOF MS) was used for an extensive identification
study of arthroconidial yeasts, using 85 reference strains from the
CBS-KNAW yeast collection and 134 clinical isolates collected from
medical centers in Qatar, Greece, and Romania. The test set included 72
strains of ascomycetous yeasts (Galactomyces, Geotrichum, Saprochaete,
and Magnusiomyces spp.) and 147 strains of basidiomycetous yeasts
(Trichosporon and Guehomyces spp.). With minimal preparation time,
MALDI-TOF MS proved to be an excellent diagnostic tool that provided
reliable identification of most (98%) of the tested strains to the
species level, with good discriminatory power. The majority of strains
were correctly identified at the species level with good scores (> 2.0)
and seven of the tested strains with log score values between 1.7 and
2.0. The MALDI-TOF MS results obtained were consistent with validated
internal transcribed spacer (ITS) and/or large subunit (LSU) ribosomal
DNA sequencing results. Expanding the mass spectrum database by
increasing the number of reference strains for closely related species,
including those of nonclinical origin, should enhance the usefulness of
MALDI-TOF MS-based diagnostic analysis of these arthroconidial fungi in
medical and other laboratories.
Συγγραφείς:
Kolecka, Anna
Khayhan, Kantarawee
Groenewald, Marizeth and
Theelen, Bart
Arabatzis, Michael
Velegraki, Aristea and
Kostrzewa, Markus
Mares, Mihai
Taj-Aldeen, Saad J.
Boekhout,
Teun
Περιοδικό:
Journal of Clinical Microbiology
Εκδότης:
AMER SOC MICROBIOLOGY
DOI:
10.1128/JCM.00470-13
