Τίτλος:
Comparative Assessment of Lymph Node Micrometastasis in Cervical,
Endometrial and Vulvar Cancer: Insights on the Real Time qRT-PCR
Approach versus Immunohistochemistry, Employing Dual Molecular Markers
Περίληψη:
To address the value of qRT-PCR and IHC in accurately detecting lymph
node micrometastasis in gynecological cancer, we performed a systematic
approach, using a set of dual molecular tumor-specific markers such as
cytokeratin 19 (CK19) and carbonic anhydrase 9 (CA9), in a series of 46
patients (19 with cervical cancer, 18 with endometrial cancer, and 9
with vulvar cancer). A total of 1281 lymph nodes were analyzed and 28
were found positive by histopathology. Following this documentation, 82
lymph nodes, 11 positive and 71 negative, were randomly selected and
further analyzed both by IHC and qRT-PCR for CK19 and CA9 expression.
All 11 (100%) expressed CK19 by IHC, while only 6 (54.5%) expressed
CA9. On the contrary, all the histologically negative for
micrometastases lymph nodes were also negative by IHC analysis for both
markers. The comparative diagnostic efficacy of the two markers using
qRT-PCR, however, disclosed that the analysis of the same aliquots of
the 82 lymph nodes led to 100% specificity for the CK19 biomarker,
while, in contrast, CA9 failed to recapitulate a similar pattern. These
data suggest that qRT-PCR exhibits a better diagnostic accuracy compared
to IHC, while CK19 displays a consistent pattern of detection compared
to CA9.
Συγγραφείς:
Pappa, Kalliopi I.
Rodolakis, Alexandros
Christodoulou, Ioanna
and Gazouli, Maria
Markaki, Sofia
Antsaklis, Aris
Anagnou,
Nicholas P.
