Τίτλος:
Development and validation of a multiplex PCR assay for identification
of the epidemic ST-258/512 KPC-producing Klebsiella pneumoniae clone
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The Klebsiella pneumoniae carbapenemase-producing Klebsiella pneumoniae
(KPC-KP) sequence type (ST)-258/512 clone is the dominant clone by which
KPC has disseminated worldwide. Standard typing methods are
time-consuming and are therefore impractical for identification of this
clone in the course of an outbreak. Through comparative genomic study,
we have previously identified several presumably unique genes of this
clone: 1) PILV-like protein (pilv-l), 2) transposase, IS66-family
(is-66), and a 3) phage-related protein (pip). Our aims were to 1) test
for the presence of these genes using a multiplex PCR in a large,
multinational collection of KPC-KP isolates and to 2) validate this
assay as a typing method for the identification of the ST-258/512 clone.
KPC-KP isolates (n = 160) that included both ST-258/512 (group A, n =
114) and non-ST-258 (group B, n = 46) strains were collected from the
following countries: Greece, 20; Israel, 93; Italy, 19; USA, 25; and
Colombia, 3. Group B included 30 different STs from various lineages.
The pilv-l gene was present in 111/114 of ST-258 isolates, including all
of the KPC-negative isolates resulting in a sensitivity of 97%. Using
primers for a unique ST-258 pilv-l allele resulted in a specificity of
100%. The sensitivity values of is-66 and pip genes for detecting
KPC-KP ST-258 were 83 and 89%, respectively, and the specificity values
were 67 and 93%, respectively. PCR for the unique pilv-l ST-258 allele
provides a reliable tool for rapid detection of the ST-258 clone. This
method can be helpful both in the setting of an outbreak and in a
large-scale survey of KPC-KP strains. (C) 2014 Elsevier Inc. All rights
reserved.
Συγγραφείς:
Adler, Amos
Khabra, Efrat
Chmelnitsky, Inna
Giakkoupi,
Panagiota
Vatopoulos, Alkiviadis
Mathers, Amy J.
Yeh,
Anthony J.
Sifri, Costi D.
De Angelis, Giulia
Tacconelli,
Evelina
Villegas, Maria-Virginia
Quinn, John
Carmeli, Yehuda
Περιοδικό:
Diagnostic Microbiology and Infectious Disease
Εκδότης:
EXCERPTA MEDICA INC-ELSEVIER SCIENCE INC
Λέξεις-κλειδιά:
KPC; Epidemic clone; Typing
DOI:
10.1016/j.diagmicrobio.2013.10.003
