Τίτλος:
CEND1 and NEUROGENIN2 Reprogram Mouse Astrocytes and Embryonic
Fibroblasts to Induced Neural Precursors and Differentiated Neurons
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Recent studies demonstrate that astroglia from non-neurogenic brain
regions can be reprogrammed into functional neurons through forced
expression of neurogenic factors. Here we explored the effect of CEND1
and NEUROG2 on reprogramming of mouse cortical astrocytes and embryonic
fibroblasts. Forced expression of CEND1, NEUROG2, or both resulted in
acquisition of induced neuronal cells expressing subtype-specific
markers, while long-term live-cell imaging highlighted the existence of
two different modes of neuronal trans-differentiation. Of note, a
subpopulation of CEND1 and NEUROG2 double-transduced astrocytes formed
spheres exhibiting neural stem cell properties. mRNA and protein
expression studies revealed a reciprocal feedback loop existing between
the two molecules, while knockdown of endogenous CEND1 demonstrated that
it is a key mediator of NEUROG2-driven neuronal reprogramming. Our data
suggest that common reprogramming mechanisms exist driving the
conversion of lineage-distant somatic cell types to neurons and reveal a
critical role for CEND1 in NEUROG2-driven astrocytic reprogramming.
Συγγραφείς:
Aravantinou-Fatorou, Katerina
Ortega, Felipe
Chroni-Tzartou,
Dafni
Antoniou, Nasia
Poulopoulou, Cornelia
Politis,
Panagiotis K.
Berninger, Benedikt
Matsas, Rebecca
Thomaidou,
Dimitra
Περιοδικό:
Current Stem Cell Reports
DOI:
10.1016/j.stemcr.2015.07.012
