Περίληψη:
Purpose To evaluate the in vitro efficacy of several anti-staphylococcal
agents against a nationwide collection of contemporary Staphylococcus
aureus clinical isolates from several healthcare centres in Greece.
Methods Thirty hospitals throughout Greece (18 in Attica) provided all
clinical isolates of S.aureus from April 2012 to May 2013 to a central
lab to be re-submitted to susceptibility testing. The MICs were
evaluated by Vitek (R) 2 with the exception of ceftaroline (OXOID M.I.C.
Evaluator). Vancomycin and daptomycin MICs were also evaluated by Etest
(R). Heterogeneously vancomycin-intermediate strains (hVISA) were
detected by the Etest (R) GRD. VISA phenotype was confirmed by PAP-AUC.
Results A total of 1005 isolates (39% MRSA) were studied.
Susceptibility rates were: erythromycin 66.5%, clindamycin 79.2%, SXT
98.9%, rifampicin 97.3%, fusidic acid 67%, moxifloxacin 78.8%,
vancomycin 99.9%, ceftaroline 92.9% and linezolid, tigecycline and
daptomycin 100%. For mupirocin, high level resistance could be excluded
for 98.9% of isolates. Vancomycin Etest (R) MIC50/90 were 1.5/1.5mg/L,
58.5% of isolates exhibited a MIC>1 and 8.7% a MIC of 2mg/L, while
Vitek (R) MIC50/90 were 1/1 and 3.1% showed MIC>1mg/L. One VISA strain
was detected. Among the selected 175 isolates that were screened for
hVISA phenotype, six (3.4%) were positive. In 315 bloodstream isolates,
64.1% had a vancomycin Etest (R) MIC>1mg/L. Conclusions This
multi-centre surveillance study revealed that a significant percentage
of contemporary S.aureus isolates from Greek patients have a vancomycin
MIC (>1mg/L) that may compromise the clinical efficacy of the drug for
the treatment of serious infections. The in vitro activity of SXT,
rifampicin, mupirocin, linezolid, tigecycline, daptomycin and
ceftaroline remains excellent.
Συγγραφείς:
Souli, Maria
Karaiskos, Ilias
Galani, Lambrini
Maraki, Sofia
and Perivolioti, Efstathia
Argyropoulou, Athina
Charissiadou,
Athina
Zachariadou, Levantia
Tsiplakou, Sofia
Papaioannou,
Vassiliki
Tsorlini, Helen
Katsifa, Helen
Baka, Vasiliki and
Pantazi, Paraskevi
Paschali, Angeliki
Kyratsa, Anna and
Trikka-Graphakos, Eleftheria
Giannopoulou, Panagiota and
Vogiatzakis, Evangelos
Moraitou, Helen
Papadogeorgaki, Helen and
Avgerinou, Helen
Panagea, Theofano
Pantazatou, Angeliki and
Petinaki, Efthymia
Stamatopoulou, Giannoula
Toutouza, Marina and
Karatzoglou, Ioanna
Kontopoulou, Konstantina
Orfanidou, Maria
and Karantani, Irene
Fytas, Panteleimon
Tzanetou, Konstantina
and Platsouka, Evangelia
Kazila, Polyzo
Chli, Anastasia and
Statiri, Ntina
Giamarellou, Helen
